5JN8
Crystal Structure for the complex of human carbonic anhydrase IV and acetazolamide
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004089 | molecular_function | carbonate dehydratase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005791 | cellular_component | rough endoplasmic reticulum |
| A | 0005793 | cellular_component | endoplasmic reticulum-Golgi intermediate compartment |
| A | 0005794 | cellular_component | Golgi apparatus |
| A | 0005802 | cellular_component | trans-Golgi network |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0009897 | cellular_component | external side of plasma membrane |
| A | 0009986 | cellular_component | cell surface |
| A | 0015701 | biological_process | bicarbonate transport |
| A | 0016020 | cellular_component | membrane |
| A | 0016323 | cellular_component | basolateral plasma membrane |
| A | 0016324 | cellular_component | apical plasma membrane |
| A | 0016829 | molecular_function | lyase activity |
| A | 0030658 | cellular_component | transport vesicle membrane |
| A | 0030667 | cellular_component | secretory granule membrane |
| A | 0031526 | cellular_component | brush border membrane |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048471 | cellular_component | perinuclear region of cytoplasm |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0098552 | cellular_component | side of membrane |
| B | 0004089 | molecular_function | carbonate dehydratase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005791 | cellular_component | rough endoplasmic reticulum |
| B | 0005793 | cellular_component | endoplasmic reticulum-Golgi intermediate compartment |
| B | 0005794 | cellular_component | Golgi apparatus |
| B | 0005802 | cellular_component | trans-Golgi network |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0009897 | cellular_component | external side of plasma membrane |
| B | 0009986 | cellular_component | cell surface |
| B | 0015701 | biological_process | bicarbonate transport |
| B | 0016020 | cellular_component | membrane |
| B | 0016323 | cellular_component | basolateral plasma membrane |
| B | 0016324 | cellular_component | apical plasma membrane |
| B | 0016829 | molecular_function | lyase activity |
| B | 0030658 | cellular_component | transport vesicle membrane |
| B | 0030667 | cellular_component | secretory granule membrane |
| B | 0031526 | cellular_component | brush border membrane |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0048471 | cellular_component | perinuclear region of cytoplasm |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0098552 | cellular_component | side of membrane |
| C | 0004089 | molecular_function | carbonate dehydratase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005791 | cellular_component | rough endoplasmic reticulum |
| C | 0005793 | cellular_component | endoplasmic reticulum-Golgi intermediate compartment |
| C | 0005794 | cellular_component | Golgi apparatus |
| C | 0005802 | cellular_component | trans-Golgi network |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0006730 | biological_process | one-carbon metabolic process |
| C | 0008270 | molecular_function | zinc ion binding |
| C | 0009897 | cellular_component | external side of plasma membrane |
| C | 0009986 | cellular_component | cell surface |
| C | 0015701 | biological_process | bicarbonate transport |
| C | 0016020 | cellular_component | membrane |
| C | 0016323 | cellular_component | basolateral plasma membrane |
| C | 0016324 | cellular_component | apical plasma membrane |
| C | 0016829 | molecular_function | lyase activity |
| C | 0030658 | cellular_component | transport vesicle membrane |
| C | 0030667 | cellular_component | secretory granule membrane |
| C | 0031526 | cellular_component | brush border membrane |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0048471 | cellular_component | perinuclear region of cytoplasm |
| C | 0070062 | cellular_component | extracellular exosome |
| C | 0098552 | cellular_component | side of membrane |
| D | 0004089 | molecular_function | carbonate dehydratase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005791 | cellular_component | rough endoplasmic reticulum |
| D | 0005793 | cellular_component | endoplasmic reticulum-Golgi intermediate compartment |
| D | 0005794 | cellular_component | Golgi apparatus |
| D | 0005802 | cellular_component | trans-Golgi network |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0006730 | biological_process | one-carbon metabolic process |
| D | 0008270 | molecular_function | zinc ion binding |
| D | 0009897 | cellular_component | external side of plasma membrane |
| D | 0009986 | cellular_component | cell surface |
| D | 0015701 | biological_process | bicarbonate transport |
| D | 0016020 | cellular_component | membrane |
| D | 0016323 | cellular_component | basolateral plasma membrane |
| D | 0016324 | cellular_component | apical plasma membrane |
| D | 0016829 | molecular_function | lyase activity |
| D | 0030658 | cellular_component | transport vesicle membrane |
| D | 0030667 | cellular_component | secretory granule membrane |
| D | 0031526 | cellular_component | brush border membrane |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0048471 | cellular_component | perinuclear region of cytoplasm |
| D | 0070062 | cellular_component | extracellular exosome |
| D | 0098552 | cellular_component | side of membrane |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 10 |
| Details | binding site for residue AZM A 701 |
| Chain | Residue |
| A | GLN92 |
| A | SO4705 |
| A | HIS94 |
| A | HIS96 |
| A | HIS119 |
| A | LEU198 |
| A | THR199 |
| A | THR200 |
| A | TRP209 |
| A | ZN702 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 702 |
| Chain | Residue |
| A | HIS94 |
| A | HIS96 |
| A | HIS119 |
| A | AZM701 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 A 703 |
| Chain | Residue |
| A | ARG27 |
| A | LEU195 |
| A | ARG254 |
| A | THR255 |
| A | ACT708 |
| A | HOH834 |
| A | HOH884 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 A 704 |
| Chain | Residue |
| A | GLY50 |
| A | TYR51 |
| A | ASP52 |
| A | LYS53 |
| A | HOH838 |
| A | HOH898 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 A 705 |
| Chain | Residue |
| A | ASN62 |
| A | HIS64 |
| A | GLN92 |
| A | HIS94 |
| A | AZM701 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 706 |
| Chain | Residue |
| A | ARG46 |
| A | GLU187 |
| C | ASN72 |
| C | LYS91 |
| site_id | AC7 |
| Number of Residues | 9 |
| Details | binding site for residue ACT A 707 |
| Chain | Residue |
| A | SER98 |
| A | ASP99 |
| A | LEU100 |
| A | PRO101 |
| A | ALA115 |
| A | ALA150 |
| A | ILE223 |
| A | LEU224 |
| A | HOH861 |
| site_id | AC8 |
| Number of Residues | 2 |
| Details | binding site for residue ACT A 708 |
| Chain | Residue |
| A | THR255 |
| A | SO4703 |
| site_id | AC9 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 709 |
| Chain | Residue |
| A | PHE48 |
| A | SER50 |
| A | SER78 |
| A | ILE79 |
| A | SER80 |
| A | PRO87 |
| C | GLN60 |
| site_id | AD1 |
| Number of Residues | 12 |
| Details | binding site for residue AZM B 701 |
| Chain | Residue |
| B | GLN92 |
| B | HIS94 |
| B | HIS96 |
| B | HIS119 |
| B | LEU198 |
| B | THR199 |
| B | THR200 |
| B | ZN702 |
| B | SO4706 |
| B | HOH802 |
| B | HOH842 |
| B | HOH876 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue ZN B 702 |
| Chain | Residue |
| B | HIS94 |
| B | HIS96 |
| B | HIS119 |
| B | AZM701 |
| site_id | AD3 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 B 703 |
| Chain | Residue |
| B | ARG27 |
| B | LEU195 |
| B | ARG254 |
| B | THR255 |
| B | HOH806 |
| B | HOH845 |
| B | HOH886 |
| site_id | AD4 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 B 704 |
| Chain | Residue |
| B | GLY50 |
| B | TYR51 |
| B | ASP52 |
| B | LYS53 |
| B | HOH803 |
| B | HOH907 |
| B | HOH944 |
| site_id | AD5 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 B 705 |
| Chain | Residue |
| B | LYS233 |
| C | GLY45 |
| C | ARG46 |
| C | ARG189 |
| site_id | AD6 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 B 706 |
| Chain | Residue |
| B | ASN62 |
| B | HIS94 |
| B | AZM701 |
| B | HOH864 |
| site_id | AD7 |
| Number of Residues | 5 |
| Details | binding site for residue ACT B 707 |
| Chain | Residue |
| B | LYS15 |
| D | GLU155 |
| D | GLN158 |
| D | GLU162 |
| D | HOH802 |
| site_id | AD8 |
| Number of Residues | 8 |
| Details | binding site for residue GOL B 708 |
| Chain | Residue |
| B | ILE79 |
| B | SER80 |
| B | PRO87 |
| B | HOH859 |
| D | GLN60 |
| D | MET67 |
| B | PHE48 |
| B | SER78 |
| site_id | AD9 |
| Number of Residues | 5 |
| Details | binding site for residue GOL B 709 |
| Chain | Residue |
| B | ARG193 |
| B | TYR194 |
| B | THR255 |
| B | HOH884 |
| B | HOH886 |
| site_id | AE1 |
| Number of Residues | 9 |
| Details | binding site for residue AZM C 701 |
| Chain | Residue |
| C | GLN92 |
| C | HIS94 |
| C | HIS96 |
| C | HIS119 |
| C | LEU198 |
| C | THR199 |
| C | THR200 |
| C | TRP209 |
| C | ZN702 |
| site_id | AE2 |
| Number of Residues | 4 |
| Details | binding site for residue ZN C 702 |
| Chain | Residue |
| C | HIS94 |
| C | HIS96 |
| C | HIS119 |
| C | AZM701 |
| site_id | AE3 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 C 703 |
| Chain | Residue |
| C | ARG27 |
| C | LEU195 |
| C | ARG254 |
| C | THR255 |
| C | HOH838 |
| C | HOH839 |
| site_id | AE4 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 C 704 |
| Chain | Residue |
| C | GLY50 |
| C | TYR51 |
| C | ASP52 |
| C | LYS53 |
| C | HOH808 |
| C | HOH927 |
| C | HOH941 |
| site_id | AE5 |
| Number of Residues | 4 |
| Details | binding site for residue ACT C 705 |
| Chain | Residue |
| A | ARG46 |
| A | ARG189 |
| C | ASN72 |
| C | GLN89 |
| site_id | AE6 |
| Number of Residues | 8 |
| Details | binding site for residue GOL C 706 |
| Chain | Residue |
| C | LYS103 |
| C | ASP243 |
| C | VAL245 |
| C | PRO247 |
| C | HOH818 |
| C | HOH905 |
| C | HOH937 |
| C | HOH955 |
| site_id | AE7 |
| Number of Residues | 4 |
| Details | binding site for residue GOL C 707 |
| Chain | Residue |
| C | SER50 |
| C | ASN72 |
| C | SER78 |
| C | GLN89 |
| site_id | AE8 |
| Number of Residues | 13 |
| Details | binding site for residue AZM D 701 |
| Chain | Residue |
| D | GLN92 |
| D | HIS94 |
| D | HIS96 |
| D | HIS119 |
| D | VAL121 |
| D | LEU198 |
| D | THR199 |
| D | THR200 |
| D | TRP209 |
| D | ZN702 |
| D | HOH842 |
| D | HOH871 |
| D | HOH1016 |
| site_id | AE9 |
| Number of Residues | 4 |
| Details | binding site for residue ZN D 702 |
| Chain | Residue |
| D | HIS94 |
| D | HIS96 |
| D | HIS119 |
| D | AZM701 |
| site_id | AF1 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 D 703 |
| Chain | Residue |
| D | ARG27 |
| D | ARG254 |
| D | THR255 |
| D | HOH835 |
| D | HOH853 |
| site_id | AF2 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 D 704 |
| Chain | Residue |
| A | GLU2 |
| D | GLY50 |
| D | TYR51 |
| D | ASP52 |
| D | LYS53 |
| site_id | AF3 |
| Number of Residues | 8 |
| Details | binding site for residue ACT D 705 |
| Chain | Residue |
| D | ASP99 |
| D | LEU100 |
| D | PRO101 |
| D | ALA115 |
| D | ALA150 |
| D | ILE223 |
| D | LEU224 |
| D | HOH815 |
Functional Information from PROSITE/UniProt
| site_id | PS00162 |
| Number of Residues | 17 |
| Details | ALPHA_CA_1 Alpha-carbonic anhydrases signature. SEHsLdgehFamEMHIV |
| Chain | Residue | Details |
| A | SER105-VAL121 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000250|UniProtKB:P00918 |
| Chain | Residue | Details |
| A | HIS64 | |
| B | HIS64 | |
| C | HIS64 | |
| D | HIS64 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:8942978 |
| Chain | Residue | Details |
| A | HIS94 | |
| A | HIS96 | |
| A | HIS119 | |
| B | HIS94 | |
| B | HIS96 | |
| B | HIS119 | |
| C | HIS94 | |
| C | HIS96 | |
| C | HIS119 | |
| D | HIS94 | |
| D | HIS96 | |
| D | HIS119 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P00918 |
| Chain | Residue | Details |
| A | THR199 | |
| B | THR199 | |
| C | THR199 | |
| D | THR199 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | LIPID: GPI-anchor amidated serine => ECO:0000269|PubMed:7625839 |
| Chain | Residue | Details |
| A | SER259 | |
| B | SER259 | |
| C | SER259 | |
| D | SER259 |
