4WDR

Crystal structure of haloalkane dehalogenase LinB 140A+143L+177W+211L mutant (LinB86) from Sphingobium japonicum UT26

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0009636	biological_process	response to toxic substance
A	0016787	molecular_function	hydrolase activity
A	0018786	molecular_function	haloalkane dehalogenase activity
A	0042597	cellular_component	periplasmic space
B	0003824	molecular_function	catalytic activity
B	0009636	biological_process	response to toxic substance
B	0016787	molecular_function	hydrolase activity
B	0018786	molecular_function	haloalkane dehalogenase activity
B	0042597	cellular_component	periplasmic space

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue CL A 301

Chain	Residue
A	ASN38
A	TRP109
A	PRO208

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site_id	AC2
Number of Residues	3
Details	binding site for residue CA A 302

Chain	Residue
A	ASP73
A	ASP73
A	ASP73

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Nucleophile => ECO:0000305|PubMed:10100638, ECO:0000305|PubMed:12939138, ECO:0000305|PubMed:14744129

Chain	Residue	Details
A	ASP108
B	ASP108

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site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Proton donor => ECO:0000305|PubMed:10100638, ECO:0000305|PubMed:12939138, ECO:0000305|PubMed:14744129

Chain	Residue	Details
B	GLU132
A	GLU132

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site_id	SWS_FT_FI3
Number of Residues	2
Details	ACT_SITE: Proton acceptor => ECO:0000305|PubMed:10100638, ECO:0000305|PubMed:12939138, ECO:0000305|PubMed:14744129

Chain	Residue	Details
A	HIS272
B	HIS272

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site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:14744129

Chain	Residue	Details
A	ASN38
B	ASN38

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site_id	SWS_FT_FI5
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:14744129, ECO:0000269|PubMed:17259360, ECO:0007744|PDB:1MJ5, ECO:0007744|PDB:2BFN

Chain	Residue	Details
A	TRP109
B	TRP109

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 467

Chain	Residue	Details
A	ASN38	electrostatic stabiliser
A	ASP108	covalent catalysis
A	TRP109	electrostatic stabiliser
A	GLU132	activator, electrostatic stabiliser
A	HIS272	activator, electrostatic stabiliser, proton shuttle (general acid/base)

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site_id	MCSA2
Number of Residues	5
Details	M-CSA 467

Chain	Residue	Details
B	ASN38	electrostatic stabiliser
B	ASP108	covalent catalysis
B	TRP109	electrostatic stabiliser
B	GLU132	activator, electrostatic stabiliser
B	HIS272	activator, electrostatic stabiliser, proton shuttle (general acid/base)

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