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4WB7

Crystal structure of a chimeric fusion of human DnaJ (Hsp40) and cAMP-dependent protein kinase A (catalytic alpha subunit)

Functional Information from GO Data
ChainGOidnamespacecontents
A0000166molecular_functionnucleotide binding
A0000287molecular_functionmagnesium ion binding
A0001669cellular_componentacrosomal vesicle
A0001707biological_processmesoderm formation
A0001843biological_processneural tube closure
A0002027biological_processregulation of heart rate
A0003091biological_processrenal water homeostasis
A0004672molecular_functionprotein kinase activity
A0004674molecular_functionprotein serine/threonine kinase activity
A0004679molecular_functionAMP-activated protein kinase activity
A0004691molecular_functioncAMP-dependent protein kinase activity
A0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005634cellular_componentnucleus
A0005654cellular_componentnucleoplasm
A0005737cellular_componentcytoplasm
A0005739cellular_componentmitochondrion
A0005759cellular_componentmitochondrial matrix
A0005813cellular_componentcentrosome
A0005829cellular_componentcytosol
A0005886cellular_componentplasma membrane
A0005929cellular_componentcilium
A0005930cellular_componentaxoneme
A0005952cellular_componentcAMP-dependent protein kinase complex
A0006397biological_processmRNA processing
A0006468biological_processprotein phosphorylation
A0006611biological_processprotein export from nucleus
A0007189biological_processadenylate cyclase-activating G protein-coupled receptor signaling pathway
A0010737biological_processprotein kinase A signaling
A0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
A0016020cellular_componentmembrane
A0016241biological_processregulation of macroautophagy
A0016301molecular_functionkinase activity
A0016310biological_processphosphorylation
A0016607cellular_componentnuclear speck
A0016740molecular_functiontransferase activity
A0018105biological_processpeptidyl-serine phosphorylation
A0019221biological_processcytokine-mediated signaling pathway
A0019901molecular_functionprotein kinase binding
A0019904molecular_functionprotein domain specific binding
A0030145molecular_functionmanganese ion binding
A0031410cellular_componentcytoplasmic vesicle
A0031514cellular_componentmotile cilium
A0031594cellular_componentneuromuscular junction
A0031625molecular_functionubiquitin protein ligase binding
A0032024biological_processpositive regulation of insulin secretion
A0034237molecular_functionprotein kinase A regulatory subunit binding
A0034380biological_processhigh-density lipoprotein particle assembly
A0034605biological_processcellular response to heat
A0034704cellular_componentcalcium channel complex
A0035584biological_processobsolete calcium-mediated signaling using intracellular calcium source
A0035694biological_processmitochondrial protein catabolic process
A0036126cellular_componentsperm flagellum
A0042995cellular_componentcell projection
A0043197cellular_componentdendritic spine
A0043393biological_processregulation of protein binding
A0044853cellular_componentplasma membrane raft
A0045667biological_processregulation of osteoblast differentiation
A0045722biological_processpositive regulation of gluconeogenesis
A0045879biological_processnegative regulation of smoothened signaling pathway
A0046827biological_processpositive regulation of protein export from nucleus
A0048240biological_processsperm capacitation
A0048471cellular_componentperinuclear region of cytoplasm
A0050804biological_processmodulation of chemical synaptic transmission
A0051480biological_processregulation of cytosolic calcium ion concentration
A0051726biological_processregulation of cell cycle
A0055117biological_processregulation of cardiac muscle contraction
A0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
A0061136biological_processregulation of proteasomal protein catabolic process
A0070062cellular_componentextracellular exosome
A0070417biological_processcellular response to cold
A0070613biological_processregulation of protein processing
A0071333biological_processcellular response to glucose stimulus
A0071374biological_processcellular response to parathyroid hormone stimulus
A0071377biological_processcellular response to glucagon stimulus
A0071872biological_processcellular response to epinephrine stimulus
A0086064biological_processcell communication by electrical coupling involved in cardiac conduction
A0097546cellular_componentciliary base
A0098978cellular_componentglutamatergic synapse
A0099170biological_processpostsynaptic modulation of chemical synaptic transmission
A0106310molecular_functionprotein serine kinase activity
A1903779biological_processregulation of cardiac conduction
A1904262biological_processnegative regulation of TORC1 signaling
A1904539biological_processnegative regulation of glycolytic process through fructose-6-phosphate
A1990044biological_processprotein localization to lipid droplet
A2000810biological_processregulation of bicellular tight junction assembly
B0000166molecular_functionnucleotide binding
B0000287molecular_functionmagnesium ion binding
B0001669cellular_componentacrosomal vesicle
B0001707biological_processmesoderm formation
B0001843biological_processneural tube closure
B0002027biological_processregulation of heart rate
B0003091biological_processrenal water homeostasis
B0004672molecular_functionprotein kinase activity
B0004674molecular_functionprotein serine/threonine kinase activity
B0004679molecular_functionAMP-activated protein kinase activity
B0004691molecular_functioncAMP-dependent protein kinase activity
B0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005634cellular_componentnucleus
B0005654cellular_componentnucleoplasm
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005759cellular_componentmitochondrial matrix
B0005813cellular_componentcentrosome
B0005829cellular_componentcytosol
B0005886cellular_componentplasma membrane
B0005929cellular_componentcilium
B0005930cellular_componentaxoneme
B0005952cellular_componentcAMP-dependent protein kinase complex
B0006397biological_processmRNA processing
B0006468biological_processprotein phosphorylation
B0006611biological_processprotein export from nucleus
B0007189biological_processadenylate cyclase-activating G protein-coupled receptor signaling pathway
B0010737biological_processprotein kinase A signaling
B0010881biological_processregulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
B0016020cellular_componentmembrane
B0016241biological_processregulation of macroautophagy
B0016301molecular_functionkinase activity
B0016310biological_processphosphorylation
B0016607cellular_componentnuclear speck
B0016740molecular_functiontransferase activity
B0018105biological_processpeptidyl-serine phosphorylation
B0019221biological_processcytokine-mediated signaling pathway
B0019901molecular_functionprotein kinase binding
B0019904molecular_functionprotein domain specific binding
B0030145molecular_functionmanganese ion binding
B0031410cellular_componentcytoplasmic vesicle
B0031514cellular_componentmotile cilium
B0031594cellular_componentneuromuscular junction
B0031625molecular_functionubiquitin protein ligase binding
B0032024biological_processpositive regulation of insulin secretion
B0034237molecular_functionprotein kinase A regulatory subunit binding
B0034380biological_processhigh-density lipoprotein particle assembly
B0034605biological_processcellular response to heat
B0034704cellular_componentcalcium channel complex
B0035584biological_processobsolete calcium-mediated signaling using intracellular calcium source
B0035694biological_processmitochondrial protein catabolic process
B0036126cellular_componentsperm flagellum
B0042995cellular_componentcell projection
B0043197cellular_componentdendritic spine
B0043393biological_processregulation of protein binding
B0044853cellular_componentplasma membrane raft
B0045667biological_processregulation of osteoblast differentiation
B0045722biological_processpositive regulation of gluconeogenesis
B0045879biological_processnegative regulation of smoothened signaling pathway
B0046827biological_processpositive regulation of protein export from nucleus
B0048240biological_processsperm capacitation
B0048471cellular_componentperinuclear region of cytoplasm
B0050804biological_processmodulation of chemical synaptic transmission
B0051480biological_processregulation of cytosolic calcium ion concentration
B0051726biological_processregulation of cell cycle
B0055117biological_processregulation of cardiac muscle contraction
B0060314biological_processregulation of ryanodine-sensitive calcium-release channel activity
B0061136biological_processregulation of proteasomal protein catabolic process
B0070062cellular_componentextracellular exosome
B0070417biological_processcellular response to cold
B0070613biological_processregulation of protein processing
B0071333biological_processcellular response to glucose stimulus
B0071374biological_processcellular response to parathyroid hormone stimulus
B0071377biological_processcellular response to glucagon stimulus
B0071872biological_processcellular response to epinephrine stimulus
B0086064biological_processcell communication by electrical coupling involved in cardiac conduction
B0097546cellular_componentciliary base
B0098978cellular_componentglutamatergic synapse
B0099170biological_processpostsynaptic modulation of chemical synaptic transmission
B0106310molecular_functionprotein serine kinase activity
B1903779biological_processregulation of cardiac conduction
B1904262biological_processnegative regulation of TORC1 signaling
B1904539biological_processnegative regulation of glycolytic process through fructose-6-phosphate
B1990044biological_processprotein localization to lipid droplet
B2000810biological_processregulation of bicellular tight junction assembly
I0004862molecular_functioncAMP-dependent protein kinase inhibitor activity
I0006469biological_processnegative regulation of protein kinase activity
J0004862molecular_functioncAMP-dependent protein kinase inhibitor activity
J0006469biological_processnegative regulation of protein kinase activity
Functional Information from PDB Data
site_idAC1
Number of Residues30
Detailsbinding site for residue ATP A 501
ChainResidue
AGLY105
AMET175
AGLU176
AVAL178
AGLU182
AASP221
ALYS223
AGLU225
AASN226
ALEU228
ATHR238
AGLY107
AASP239
APHE382
AZN502
AZN503
AHOH706
AHOH734
AHOH735
AHOH819
IARG18
IASN20
ASER108
IALA21
APHE109
AGLY110
AVAL112
AALA125
ALYS127
AVAL159

site_idAC2
Number of Residues4
Detailsbinding site for residue ZN A 502
ChainResidue
AASN226
AASP239
AATP501
AHOH706

site_idAC3
Number of Residues4
Detailsbinding site for residue ZN A 503
ChainResidue
AASP239
AATP501
AHOH734
AHOH735

site_idAC4
Number of Residues5
Detailsbinding site for residue ZN A 504
ChainResidue
AGLU79
AASP80
AHOH606
BHIS94
BASP96

site_idAC5
Number of Residues4
Detailsbinding site for residue ZN A 505
ChainResidue
ASEP194
AHIS197
AHOH1065
JHOH209

site_idAC6
Number of Residues4
Detailsbinding site for residue ZN A 506
ChainResidue
AGLU176
AGLN236
AHOH905
AHOH912

site_idAC7
Number of Residues3
Detailsbinding site for residue ZN A 507
ChainResidue
AHIS123
AHOH916
AHOH1010

site_idAC8
Number of Residues30
Detailsbinding site for residue ATP B 501
ChainResidue
BGLY105
BGLY107
BSER108
BPHE109
BGLY110
BVAL112
BALA125
BLYS127
BVAL159
BMET175
BGLU176
BVAL178
BGLU182
BASP221
BLYS223
BGLU225
BASN226
BLEU228
BTHR238
BASP239
BPHE382
BZN502
BZN503
BHOH720
BHOH774
BHOH775
BHOH906
JARG18
JASN20
JALA21

site_idAC9
Number of Residues4
Detailsbinding site for residue ZN B 502
ChainResidue
BASN226
BASP239
BATP501
BHOH906

site_idAD1
Number of Residues4
Detailsbinding site for residue ZN B 503
ChainResidue
BASP239
BATP501
BHOH774
BHOH775

site_idAD2
Number of Residues5
Detailsbinding site for residue ZN B 504
ChainResidue
AHIS94
AASP96
BGLU79
BASP80
BHOH603

site_idAD3
Number of Residues4
Detailsbinding site for residue ZN B 505
ChainResidue
BHOH633
BHOH848
BSEP194
BHIS197

site_idAD4
Number of Residues5
Detailsbinding site for residue ZN I 101
ChainResidue
IHIS23
IZN102
IZN103
JZN101
JZN102

site_idAD5
Number of Residues2
Detailsbinding site for residue ZN I 102
ChainResidue
IZN101
JZN101

site_idAD6
Number of Residues4
Detailsbinding site for residue ZN I 103
ChainResidue
IHIS23
IZN101
JHIS23
JZN101

site_idAD7
Number of Residues5
Detailsbinding site for residue ZN J 101
ChainResidue
IZN101
IZN102
IZN103
JHIS23
JZN102

site_idAD8
Number of Residues2
Detailsbinding site for residue ZN J 102
ChainResidue
IZN101
JZN101

Functional Information from PROSITE/UniProt
site_idPS00107
Number of Residues24
DetailsPROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGTGSFGRVMlVkhketgnh..........YAMK
ChainResidueDetails
ALEU104-LYS127

site_idPS00108
Number of Residues13
DetailsPROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. LiYrDLKpeNLLI
ChainResidueDetails
ALEU217-ILE229

site_idPS00636
Number of Residues20
DetailsDNAJ_1 Nt-dnaJ domain signature. FKeIAeaYDvLsDprkREIF
ChainResidueDetails
APHE44-PHE63

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues6
DetailsSITE: Important for inhibition => ECO:0000250
ChainResidueDetails
IARG15
IARG18
IARG19
JARG15
JARG18
JARG19

site_idSWS_FT_FI2
Number of Residues2
DetailsBINDING:
ChainResidueDetails
ALEU104
BLEU104

site_idSWS_FT_FI3
Number of Residues6
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159
ChainResidueDetails
ALYS127
AGLU176
ALYS223
BLYS127
BGLU176
BLYS223

site_idSWS_FT_FI4
Number of Residues2
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:18691976
ChainResidueDetails
ATHR103
BTHR103

site_idSWS_FT_FI5
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P05132
ChainResidueDetails
ASEP194
BSEP194

site_idSWS_FT_FI6
Number of Residues2
DetailsMOD_RES: Phosphothreonine => ECO:0000269|PubMed:12372837
ChainResidueDetails
ATHR250
BTHR250

site_idSWS_FT_FI7
Number of Residues2
DetailsMOD_RES: Phosphothreonine; by PDPK1 => ECO:0000269|PubMed:12372837, ECO:0000269|PubMed:16765046, ECO:0000269|PubMed:20137943, ECO:0000269|PubMed:20481595, ECO:0000269|PubMed:20732331, ECO:0000269|PubMed:21774789, ECO:0000269|Ref.43
ChainResidueDetails
ATPO252
BTPO252

site_idSWS_FT_FI8
Number of Residues2
DetailsMOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P05132
ChainResidueDetails
ATYR385
BTYR385

site_idSWS_FT_FI9
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0000269|PubMed:16765046, ECO:0000269|PubMed:20137943, ECO:0000269|PubMed:20481595, ECO:0000269|PubMed:20732331, ECO:0000269|PubMed:21774789, ECO:0000269|Ref.43, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:24275569
ChainResidueDetails
ASEP393
BSEP393

218853

PDB entries from 2024-04-24

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