4RNT

HIS 92 ALA MUTATION IN RIBONUCLEASE T1 INDUCES SEGMENTAL FLEXIBILITY. AN X-RAY STUDY

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001411	cellular_component	hyphal tip
A	0003723	molecular_function	RNA binding
A	0004519	molecular_function	endonuclease activity
A	0004540	molecular_function	RNA nuclease activity
A	0008150	biological_process	biological_process
A	0016829	molecular_function	lyase activity
A	0030428	cellular_component	cell septum
A	0046589	molecular_function	ribonuclease T1 activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:2844811

Chain	Residue	Details
A	HIS40

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site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000269|PubMed:2844811

Chain	Residue	Details
A	GLU58

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site_id	SWS_FT_FI3
Number of Residues	1
Details	ACT_SITE: Proton donor

Chain	Residue	Details
A	ALA92

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 414

Chain	Residue	Details
A	TYR38	electrostatic stabiliser
A	HIS40	proton shuttle (general acid/base)
A	GLU58	proton shuttle (general acid/base)
A	ARG77	electrostatic stabiliser
A	ALA92	proton shuttle (general acid/base)
A	PHE100	electrostatic stabiliser

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