4NCA
Structure of Thermus thermophilus Argonaute bound to guide DNA 19-mer and target DNA in the presence of Mg2+
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004520 | molecular_function | DNA endonuclease activity |
| A | 0006260 | biological_process | DNA replication |
| A | 0030145 | molecular_function | manganese ion binding |
| A | 0044355 | biological_process | clearance of foreign intracellular DNA |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0003676 | molecular_function | nucleic acid binding |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0004520 | molecular_function | DNA endonuclease activity |
| B | 0006260 | biological_process | DNA replication |
| B | 0030145 | molecular_function | manganese ion binding |
| B | 0044355 | biological_process | clearance of foreign intracellular DNA |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE DT A 701 |
| Chain | Residue |
| A | ASN195 |
| A | ILE254 |
| A | PRO255 |
| A | HIS256 |
| B | PRO37 |
| B | GLY38 |
| B | ARG39 |
| A | TYR197 |
| A | ARG200 |
| A | LEU217 |
| A | PRO218 |
| A | LEU223 |
| A | TYR226 |
| A | HIS227 |
| A | ARG232 |
| site_id | AC2 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE MG A 702 |
| Chain | Residue |
| A | ASP478 |
| A | ALA479 |
| A | ASP546 |
| A | HOH802 |
| A | HOH806 |
| D | DT10 |
| D | MG101 |
| G | DC6 |
| site_id | AC3 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE DT B 701 |
| Chain | Residue |
| A | PRO37 |
| A | GLY38 |
| A | ARG39 |
| B | ASN195 |
| B | TYR197 |
| B | ARG200 |
| B | LEU217 |
| B | PRO218 |
| B | LEU223 |
| B | TYR226 |
| B | HIS227 |
| B | ARG232 |
| B | ILE254 |
| B | PRO255 |
| B | HIS256 |
| B | HOH819 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG B 702 |
| Chain | Residue |
| B | ASP478 |
| B | ASP660 |
| B | MG703 |
| B | HOH811 |
| B | HOH895 |
| F | DT10 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE MG B 703 |
| Chain | Residue |
| B | ASP478 |
| B | ALA479 |
| B | ASP546 |
| B | MG702 |
| B | HOH805 |
| B | HOH806 |
| F | DT10 |
| H | DC9 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG C 101 |
| Chain | Residue |
| A | VAL685 |
| A | HOH801 |
| C | DT1 |
| C | DA3 |
| C | HOH201 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MG D 101 |
| Chain | Residue |
| A | ASP478 |
| A | ASP660 |
| A | MG702 |
| D | DT10 |
| D | HOH201 |
| D | HOH202 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG E 101 |
| Chain | Residue |
| B | VAL685 |
| B | HOH801 |
| E | DT1 |
| E | DA3 |
| E | HOH201 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | ACT_SITE: ACT_SITE => ECO:0000305|PubMed:18754009, ECO:0000305|PubMed:19092929, ECO:0000305|PubMed:24374628, ECO:0000305|PubMed:28911094 |
| Chain | Residue | Details |
| A | ASP478 | |
| A | ASP546 | |
| A | ASP660 | |
| B | ASP478 | |
| B | ASP546 | |
| B | ASP660 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | ACT_SITE: ACT_SITE => ECO:0000305|PubMed:24374628, ECO:0000305|PubMed:28911094 |
| Chain | Residue | Details |
| A | GLU512 | |
| B | GLU512 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:24374628, ECO:0000305|PubMed:19812667, ECO:0000305|PubMed:28911094, ECO:0007744|PDB:4KPY, ECO:0007744|PDB:4N76 |
| Chain | Residue | Details |
| A | ASP478 | |
| B | ASP478 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:24374628, ECO:0000305|PubMed:19092929, ECO:0000305|PubMed:19812667, ECO:0000305|PubMed:28911094, ECO:0007744|PDB:4KPY, ECO:0007744|PDB:4N76 |
| Chain | Residue | Details |
| A | ASP546 | |
| A | ASP660 | |
| B | ASP546 | |
| B | ASP660 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:24374628, ECO:0000305|PubMed:18754009, ECO:0000305|PubMed:19092929, ECO:0000305|PubMed:19812667, ECO:0000305|PubMed:28911094, ECO:0007744|PDB:4KPY, ECO:0007744|PDB:4N76 |
| Chain | Residue | Details |
| A | VAL685 | |
| B | VAL685 |
