4LW2

Structural changes during cysteine desulfurase CsdA and sulfur-acceptor CsdE interactions provide insight into the trans-persulfuration

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000096	biological_process	sulfur amino acid metabolic process
A	0003824	molecular_function	catalytic activity
A	0005515	molecular_function	protein binding
A	0006534	biological_process	cysteine metabolic process
A	0008826	molecular_function	cysteine sulfinate desulfinase activity
A	0009000	molecular_function	selenocysteine lyase activity
A	0016226	biological_process	iron-sulfur cluster assembly
A	0016261	biological_process	selenocysteine catabolic process
A	0016740	molecular_function	transferase activity
A	0016783	molecular_function	sulfurtransferase activity
A	0016787	molecular_function	hydrolase activity
A	0016829	molecular_function	lyase activity
A	0019448	biological_process	L-cysteine catabolic process
A	0030170	molecular_function	pyridoxal phosphate binding
A	0031071	molecular_function	cysteine desulfurase activity
A	0072348	biological_process	sulfur compound transport
A	1990221	cellular_component	L-cysteine desulfurase complex
B	0000096	biological_process	sulfur amino acid metabolic process
B	0003824	molecular_function	catalytic activity
B	0005515	molecular_function	protein binding
B	0006534	biological_process	cysteine metabolic process
B	0008826	molecular_function	cysteine sulfinate desulfinase activity
B	0009000	molecular_function	selenocysteine lyase activity
B	0016226	biological_process	iron-sulfur cluster assembly
B	0016261	biological_process	selenocysteine catabolic process
B	0016740	molecular_function	transferase activity
B	0016783	molecular_function	sulfurtransferase activity
B	0016787	molecular_function	hydrolase activity
B	0016829	molecular_function	lyase activity
B	0019448	biological_process	L-cysteine catabolic process
B	0030170	molecular_function	pyridoxal phosphate binding
B	0031071	molecular_function	cysteine desulfurase activity
B	0072348	biological_process	sulfur compound transport
B	1990221	cellular_component	L-cysteine desulfurase complex
C	0000096	biological_process	sulfur amino acid metabolic process
C	0003824	molecular_function	catalytic activity
C	0005515	molecular_function	protein binding
C	0006534	biological_process	cysteine metabolic process
C	0008826	molecular_function	cysteine sulfinate desulfinase activity
C	0009000	molecular_function	selenocysteine lyase activity
C	0016226	biological_process	iron-sulfur cluster assembly
C	0016261	biological_process	selenocysteine catabolic process
C	0016740	molecular_function	transferase activity
C	0016783	molecular_function	sulfurtransferase activity
C	0016787	molecular_function	hydrolase activity
C	0016829	molecular_function	lyase activity
C	0019448	biological_process	L-cysteine catabolic process
C	0030170	molecular_function	pyridoxal phosphate binding
C	0031071	molecular_function	cysteine desulfurase activity
C	0072348	biological_process	sulfur compound transport
C	1990221	cellular_component	L-cysteine desulfurase complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	13
Details	BINDING SITE FOR RESIDUE PLP A 501

Chain	Residue
A	THR90
A	LYS222
A	THR273
A	HOH661
A	HOH702
A	THR91
A	HIS119
A	ASN171
A	ASP196
A	ALA198
A	GLN199
A	SER219
A	HIS221

---

site_id	AC2
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 502

Chain	Residue
A	ALA26
A	ALA27
A	ASN171
A	ARG353
A	HIS357
A	ARG373
A	HOH688

---

site_id	AC3
Number of Residues	14
Details	BINDING SITE FOR RESIDUE PLP B 501

Chain	Residue
B	THR90
B	THR91
B	HIS119
B	ASN171
B	ASP196
B	ALA198
B	GLN199
B	SER219
B	HIS221
B	LYS222
B	HOH616
B	HOH662
C	THR273
C	HOH626

---

site_id	AC4
Number of Residues	11
Details	BINDING SITE FOR RESIDUE GOL B 502

Chain	Residue
B	ALA26
B	ALA27
B	ASN171
B	LYS222
B	ARG353
B	HIS357
B	ARG373
B	HOH640
B	HOH692
B	HOH913
B	HOH956

---

site_id	AC5
Number of Residues	15
Details	BINDING SITE FOR RESIDUE PLP C 501

Chain	Residue
B	GLY272
B	THR273
C	THR90
C	THR91
C	HIS119
C	ASN171
C	ASP196
C	ALA198
C	GLN199
C	SER219
C	HIS221
C	LYS222
C	HOH618
C	HOH764
C	HOH890

---

site_id	AC6
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL C 502

Chain	Residue
C	ALA26
C	ALA27
C	ASN171
C	ARG353
C	HIS357
C	ARG373
C	HOH669
C	HOH700

---

Functional Information from PROSITE/UniProt

site_id	PS00595
Number of Residues	20
Details	AA_TRANSFER_CLASS_5 Aminotransferases class-V pyridoxal-phosphate attachment site. IDFYafSGHKlygpt.GiGvL

Chain	Residue	Details
A	ILE213-LEU232

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	3
Details	ACT_SITE: Cysteine persulfide intermediate => ECO:0000305|PubMed:10739946

Chain	Residue	Details
A	CYS358
B	CYS358
C	CYS358

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000250

Chain	Residue	Details
A	LYS222
B	LYS222
C	LYS222

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