4DBL

Crystal structure of E159Q mutant of BtuCDF

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0015420	molecular_function	ABC-type vitamin B12 transporter activity
A	0015889	biological_process	cobalamin transport
A	0016020	cellular_component	membrane
A	0022857	molecular_function	transmembrane transporter activity
A	0035461	biological_process	vitamin transmembrane transport
A	0042802	molecular_function	identical protein binding
A	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
A	0055085	biological_process	transmembrane transport
A	0090482	molecular_function	vitamin transmembrane transporter activity
A	1990191	cellular_component	cobalamin transport complex
A	1990193	cellular_component	BtuCD complex
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0015420	molecular_function	ABC-type vitamin B12 transporter activity
B	0015889	biological_process	cobalamin transport
B	0016020	cellular_component	membrane
B	0022857	molecular_function	transmembrane transporter activity
B	0035461	biological_process	vitamin transmembrane transport
B	0042802	molecular_function	identical protein binding
B	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
B	0055085	biological_process	transmembrane transport
B	0090482	molecular_function	vitamin transmembrane transporter activity
B	1990191	cellular_component	cobalamin transport complex
B	1990193	cellular_component	BtuCD complex
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005886	cellular_component	plasma membrane
C	0015420	molecular_function	ABC-type vitamin B12 transporter activity
C	0015889	biological_process	cobalamin transport
C	0016020	cellular_component	membrane
C	0016887	molecular_function	ATP hydrolysis activity
C	0019898	cellular_component	extrinsic component of membrane
C	0042626	molecular_function	ATPase-coupled transmembrane transporter activity
C	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
C	0055085	biological_process	transmembrane transport
C	1990191	cellular_component	cobalamin transport complex
C	1990193	cellular_component	BtuCD complex
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005886	cellular_component	plasma membrane
D	0015420	molecular_function	ABC-type vitamin B12 transporter activity
D	0015889	biological_process	cobalamin transport
D	0016020	cellular_component	membrane
D	0016887	molecular_function	ATP hydrolysis activity
D	0019898	cellular_component	extrinsic component of membrane
D	0042626	molecular_function	ATPase-coupled transmembrane transporter activity
D	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
D	0055085	biological_process	transmembrane transport
D	1990191	cellular_component	cobalamin transport complex
D	1990193	cellular_component	BtuCD complex
E	0015889	biological_process	cobalamin transport
E	0031419	molecular_function	cobalamin binding
E	0042597	cellular_component	periplasmic space
F	0005515	molecular_function	protein binding
F	0005886	cellular_component	plasma membrane
F	0015420	molecular_function	ABC-type vitamin B12 transporter activity
F	0015889	biological_process	cobalamin transport
F	0016020	cellular_component	membrane
F	0022857	molecular_function	transmembrane transporter activity
F	0035461	biological_process	vitamin transmembrane transport
F	0042802	molecular_function	identical protein binding
F	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
F	0055085	biological_process	transmembrane transport
F	0090482	molecular_function	vitamin transmembrane transporter activity
F	1990191	cellular_component	cobalamin transport complex
F	1990193	cellular_component	BtuCD complex
G	0005515	molecular_function	protein binding
G	0005886	cellular_component	plasma membrane
G	0015420	molecular_function	ABC-type vitamin B12 transporter activity
G	0015889	biological_process	cobalamin transport
G	0016020	cellular_component	membrane
G	0022857	molecular_function	transmembrane transporter activity
G	0035461	biological_process	vitamin transmembrane transport
G	0042802	molecular_function	identical protein binding
G	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
G	0055085	biological_process	transmembrane transport
G	0090482	molecular_function	vitamin transmembrane transporter activity
G	1990191	cellular_component	cobalamin transport complex
G	1990193	cellular_component	BtuCD complex
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005886	cellular_component	plasma membrane
H	0015420	molecular_function	ABC-type vitamin B12 transporter activity
H	0015889	biological_process	cobalamin transport
H	0016020	cellular_component	membrane
H	0016887	molecular_function	ATP hydrolysis activity
H	0019898	cellular_component	extrinsic component of membrane
H	0042626	molecular_function	ATPase-coupled transmembrane transporter activity
H	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
H	0055085	biological_process	transmembrane transport
H	1990191	cellular_component	cobalamin transport complex
H	1990193	cellular_component	BtuCD complex
I	0005515	molecular_function	protein binding
I	0005524	molecular_function	ATP binding
I	0005886	cellular_component	plasma membrane
I	0015420	molecular_function	ABC-type vitamin B12 transporter activity
I	0015889	biological_process	cobalamin transport
I	0016020	cellular_component	membrane
I	0016887	molecular_function	ATP hydrolysis activity
I	0019898	cellular_component	extrinsic component of membrane
I	0042626	molecular_function	ATPase-coupled transmembrane transporter activity
I	0043190	cellular_component	ATP-binding cassette (ABC) transporter complex
I	0055085	biological_process	transmembrane transport
I	1990191	cellular_component	cobalamin transport complex
I	1990193	cellular_component	BtuCD complex
J	0015889	biological_process	cobalamin transport
J	0031419	molecular_function	cobalamin binding
J	0042597	cellular_component	periplasmic space

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PO4 C 301

Chain	Residue
C	PRO34
C	ASN35
C	GLY36
C	ALA37
C	GLY38
C	LYS39
C	SER40

---

site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 302

Chain	Residue
C	ARG217
C	ARG243

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 303

Chain	Residue
C	ARG198
C	HIS199
C	ALA200
C	HIS201
C	ARG217

---

site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 304

Chain	Residue
C	ARG202
C	ARG216

---

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE PO4 D 301

Chain	Residue
D	PRO34
D	ASN35
D	GLY36
D	ALA37
D	GLY38
D	LYS39
D	SER40

---

site_id	AC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 D 302

Chain	Residue
D	ARG202
D	ARG216

---

site_id	AC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 303

Chain	Residue
D	ARG217
D	ARG243
D	SO4304

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 D 304

Chain	Residue
D	ARG198
D	HIS199
D	HIS201
D	ARG217
D	SO4303

---

site_id	AC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 H 301

Chain	Residue
H	GLY36
H	ALA37
H	GLY38
H	LYS39
H	SER40

---

site_id	BC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 H 302

Chain	Residue
H	ARG217
H	ARG243

---

site_id	BC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 H 303

Chain	Residue
H	ARG198
H	HIS201
H	ARG217

---

site_id	BC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 H 304

Chain	Residue
H	ARG202
H	ARG216

---

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE PO4 I 301

Chain	Residue
I	GLY36
I	ALA37
I	GLY38
I	LYS39
I	SER40

---

site_id	BC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 I 302

Chain	Residue
I	ARG202
I	ARG216

---

site_id	BC6
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 I 303

Chain	Residue
I	ARG217
I	ARG243

---

site_id	BC7
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 I 304

Chain	Residue
I	ARG198
I	HIS201
I	ARG217

---

Functional Information from PROSITE/UniProt

site_id	PS00211
Number of Residues	15
Details	ABC_TRANSPORTER_1 ABC transporters family signature. LSGGEWQRVRLAAVV

Chain	Residue	Details
C	LEU127-VAL141

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000269|PubMed:12468528, ECO:0007744|PDB:1N4A

Chain	Residue	Details
E	TYR50
B	GLN207-LEU228
B	GLY268-VAL274
B	GLY325-ARG326
F	MET1-ARG10
F	GLU82-GLY92
F	HIS139-SER141
F	GLN207-LEU228
F	GLY268-VAL274
F	GLY325-ARG326
G	MET1-ARG10
J	TYR50
G	GLU82-GLY92
G	HIS139-SER141
G	GLN207-LEU228
G	GLY268-VAL274
G	GLY325-ARG326
H	GLY33
I	GLY33
A	GLY268-VAL274
A	GLY325-ARG326
B	MET1-ARG10
B	GLU82-GLY92
B	HIS139-SER141

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000269|PubMed:12468528, ECO:0000269|PubMed:12475936, ECO:0007744|PDB:1N2Z, ECO:0007744|PDB:1N4A

Chain	Residue	Details
E	ASP242
J	ASP242
F	GLN11-GLU35
G	GLN11-GLU35

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	SITE: Important for BtuC binding => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000305|PubMed:12475936

Chain	Residue	Details
E	GLU72
B	ALA297-PRO304
F	GLN36-ARG56
F	GLY108-PRO113
F	SER167-GLN190
F	ALA250-GLY257
F	ALA297-PRO304
G	GLN36-ARG56
G	GLY108-PRO113
G	SER167-GLN190
G	ALA250-GLY257
E	GLU202
G	ALA297-PRO304
J	GLU72
J	GLU202
A	ALA297-PRO304
B	GLN36-ARG56
B	GLY108-PRO113
B	SER167-GLN190
B	ALA250-GLY257

---

site_id	SWS_FT_FI4
Number of Residues	96
Details	TRANSMEM: Helical; Name=2

Chain	Residue	Details
A	LEU57-PHE81
B	LEU57-PHE81
F	LEU57-PHE81
G	LEU57-PHE81

---

site_id	SWS_FT_FI5
Number of Residues	56
Details	TRANSMEM: Helical; Name=3

Chain	Residue	Details
A	VAL93-LEU107
B	VAL93-LEU107
F	VAL93-LEU107
G	VAL93-LEU107

---

site_id	SWS_FT_FI6
Number of Residues	96
Details	TRANSMEM: Helical; Name=4

Chain	Residue	Details
A	ASN114-ARG138
B	ASN114-ARG138
F	ASN114-ARG138
G	ASN114-ARG138

---

site_id	SWS_FT_FI7
Number of Residues	96
Details	TRANSMEM: Helical; Name=5

Chain	Residue	Details
A	THR142-PHE166
B	THR142-PHE166
F	THR142-PHE166
G	THR142-PHE166

---

site_id	SWS_FT_FI8
Number of Residues	60
Details	TRANSMEM: Helical; Name=6

Chain	Residue	Details
A	SER191-SER206
B	SER191-SER206
F	SER191-SER206
G	SER191-SER206

---

site_id	SWS_FT_FI9
Number of Residues	80
Details	TRANSMEM: Helical; Name=7

Chain	Residue	Details
A	TRP229-LEU249
B	TRP229-LEU249
F	TRP229-LEU249
G	TRP229-LEU249

---

site_id	SWS_FT_FI10
Number of Residues	36
Details	TRANSMEM: Helical; Name=8

Chain	Residue	Details
A	LEU258-SER267
B	LEU258-SER267
F	LEU258-SER267
G	LEU258-SER267

---

site_id	SWS_FT_FI11
Number of Residues	84
Details	TRANSMEM: Helical; Name=9

Chain	Residue	Details
A	LEU275-LEU296
B	LEU275-LEU296
F	LEU275-LEU296
G	LEU275-LEU296

---

site_id	SWS_FT_FI12
Number of Residues	76
Details	TRANSMEM: Helical; Name=10

Chain	Residue	Details
A	ILE305-ALA324
B	ILE305-ALA324
F	ILE305-ALA324
G	ILE305-ALA324

---