4C5S

Structural Investigations into the Stereochemistry and Activity of a Phenylalanine-2,3-Aminomutase from Taxus chinensis

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005737	cellular_component	cytoplasm
A	0006558	biological_process	L-phenylalanine metabolic process
A	0006559	biological_process	L-phenylalanine catabolic process
A	0009698	biological_process	phenylpropanoid metabolic process
A	0009800	biological_process	cinnamic acid biosynthetic process
A	0009820	biological_process	alkaloid metabolic process
A	0009821	biological_process	alkaloid biosynthetic process
A	0016829	molecular_function	lyase activity
A	0016841	molecular_function	ammonia-lyase activity
A	0016853	molecular_function	isomerase activity
A	0016869	molecular_function	intramolecular aminotransferase activity
A	0042617	biological_process	paclitaxel biosynthetic process
A	0045548	molecular_function	phenylalanine ammonia-lyase activity
A	0051289	biological_process	protein homotetramerization
B	0003824	molecular_function	catalytic activity
B	0005737	cellular_component	cytoplasm
B	0006558	biological_process	L-phenylalanine metabolic process
B	0006559	biological_process	L-phenylalanine catabolic process
B	0009698	biological_process	phenylpropanoid metabolic process
B	0009800	biological_process	cinnamic acid biosynthetic process
B	0009820	biological_process	alkaloid metabolic process
B	0009821	biological_process	alkaloid biosynthetic process
B	0016829	molecular_function	lyase activity
B	0016841	molecular_function	ammonia-lyase activity
B	0016853	molecular_function	isomerase activity
B	0016869	molecular_function	intramolecular aminotransferase activity
B	0042617	biological_process	paclitaxel biosynthetic process
B	0045548	molecular_function	phenylalanine ammonia-lyase activity
B	0051289	biological_process	protein homotetramerization
C	0003824	molecular_function	catalytic activity
C	0005737	cellular_component	cytoplasm
C	0006558	biological_process	L-phenylalanine metabolic process
C	0006559	biological_process	L-phenylalanine catabolic process
C	0009698	biological_process	phenylpropanoid metabolic process
C	0009800	biological_process	cinnamic acid biosynthetic process
C	0009820	biological_process	alkaloid metabolic process
C	0009821	biological_process	alkaloid biosynthetic process
C	0016829	molecular_function	lyase activity
C	0016841	molecular_function	ammonia-lyase activity
C	0016853	molecular_function	isomerase activity
C	0016869	molecular_function	intramolecular aminotransferase activity
C	0042617	biological_process	paclitaxel biosynthetic process
C	0045548	molecular_function	phenylalanine ammonia-lyase activity
C	0051289	biological_process	protein homotetramerization
D	0003824	molecular_function	catalytic activity
D	0005737	cellular_component	cytoplasm
D	0006558	biological_process	L-phenylalanine metabolic process
D	0006559	biological_process	L-phenylalanine catabolic process
D	0009698	biological_process	phenylpropanoid metabolic process
D	0009800	biological_process	cinnamic acid biosynthetic process
D	0009820	biological_process	alkaloid metabolic process
D	0009821	biological_process	alkaloid biosynthetic process
D	0016829	molecular_function	lyase activity
D	0016841	molecular_function	ammonia-lyase activity
D	0016853	molecular_function	isomerase activity
D	0016869	molecular_function	intramolecular aminotransferase activity
D	0042617	biological_process	paclitaxel biosynthetic process
D	0045548	molecular_function	phenylalanine ammonia-lyase activity
D	0051289	biological_process	protein homotetramerization

Functional Information from PDB Data

site_id	AC1
Number of Residues	13
Details	BINDING SITE FOR RESIDUE BQ7 A 1678

Chain	Residue
A	GLY87
A	HOH2216
B	GLN319
B	TYR322
B	ARG325
A	MDO175
A	LEU227
A	ASN231
A	ASN355
A	PHE371
A	GLU455
A	HOH2028
A	HOH2030

---

site_id	AC2
Number of Residues	13
Details	BINDING SITE FOR RESIDUE BQ7 B 1678

Chain	Residue
A	GLN319
A	TYR322
A	ARG325
A	HOH2123
A	HOH2130
B	GLY87
B	MDO175
B	LEU227
B	ASN231
B	ASN355
B	PHE371
B	GLU455
B	HOH2035

---

site_id	AC3
Number of Residues	13
Details	BINDING SITE FOR RESIDUE BQ7 C 1678

Chain	Residue
C	GLY87
C	MDO175
C	LEU227
C	ASN231
C	ASN355
C	PHE371
C	GLU455
C	HOH2028
C	HOH2030
C	HOH2170
D	GLN319
D	TYR322
D	ARG325

---

site_id	AC4
Number of Residues	14
Details	BINDING SITE FOR RESIDUE BQ7 D 1678

Chain	Residue
C	GLN319
C	TYR322
C	ARG325
C	HOH2112
C	HOH2113
C	HOH2118
D	GLY87
D	LEU104
D	MDO175
D	LEU227
D	ASN231
D	ASN355
D	PHE371
D	GLU455

---

Functional Information from PROSITE/UniProt

site_id	PS00488
Number of Residues	17
Details	PAL_HISTIDASE Phenylalanine and histidine ammonia-lyases signature. GSVSASGDLiPLAyiaG

Chain	Residue	Details
A	GLY171-LEU189

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton donor/acceptor => ECO:0000269|PubMed:22113970, ECO:0000269|PubMed:24786474

Chain	Residue	Details
A	ALA80
B	ALA80
C	ALA80
D	ALA80

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	BINDING: BINDING => ECO:0000269|PubMed:24786474, ECO:0007744|PDB:4CQ5

Chain	Residue	Details
A	THR233
A	ARG321
A	SER327
A	ILE357
A	ASP460
B	THR233
B	ARG321
B	SER327
B	ILE357
B	ASP460
C	THR233
C	ARG321
C	SER327
C	ILE357
C	ASP460
D	THR233
D	ARG321
D	SER327
D	ILE357
D	ASP460

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P11544

Chain	Residue	Details
C	LEU429
C	HIS457
D	LEU429
D	HIS457
A	LEU429
A	HIS457
B	LEU429
B	HIS457

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: 2,3-didehydroalanine (Ser) => ECO:0000269|PubMed:24786474

Chain	Residue	Details
A	ASP178
B	ASP178
C	ASP178
D	ASP178

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	CROSSLNK: 5-imidazolinone (Ala-Gly) => ECO:0000269|PubMed:24786474

Chain	Residue	Details
A	MDO175
A	LEU179
B	MDO175
B	LEU179
C	MDO175
C	LEU179
D	MDO175
D	LEU179

---