4A6G
N-acyl amino acid racemase from Amycalotopsis sp. Ts-1-60: G291D- F323Y mutant in complex with N-acetyl methionine
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0009234 | biological_process | menaquinone biosynthetic process |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016853 | molecular_function | isomerase activity |
| A | 0043748 | molecular_function | O-succinylbenzoate synthase activity |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0003824 | molecular_function | catalytic activity |
| B | 0009234 | biological_process | menaquinone biosynthetic process |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016853 | molecular_function | isomerase activity |
| B | 0043748 | molecular_function | O-succinylbenzoate synthase activity |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0009234 | biological_process | menaquinone biosynthetic process |
| C | 0016829 | molecular_function | lyase activity |
| C | 0016853 | molecular_function | isomerase activity |
| C | 0043748 | molecular_function | O-succinylbenzoate synthase activity |
| C | 0046872 | molecular_function | metal ion binding |
| D | 0003824 | molecular_function | catalytic activity |
| D | 0009234 | biological_process | menaquinone biosynthetic process |
| D | 0016829 | molecular_function | lyase activity |
| D | 0016853 | molecular_function | isomerase activity |
| D | 0043748 | molecular_function | O-succinylbenzoate synthase activity |
| D | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE AME A 1369 |
| Chain | Residue |
| A | PHE19 |
| A | ASP239 |
| A | LYS263 |
| A | ASP291 |
| A | ASP316 |
| A | MG1370 |
| A | HOH2061 |
| A | PHE23 |
| A | GLN26 |
| A | SER135 |
| A | LYS161 |
| A | LYS163 |
| A | ASP189 |
| A | ASN191 |
| A | GLU214 |
| site_id | AC2 |
| Number of Residues | 16 |
| Details | BINDING SITE FOR RESIDUE AME B 1368 |
| Chain | Residue |
| B | PHE19 |
| B | MET50 |
| B | SER135 |
| B | LYS161 |
| B | LYS163 |
| B | ASP189 |
| B | ASN191 |
| B | GLU214 |
| B | ASP239 |
| B | LYS263 |
| B | ASP291 |
| B | MET292 |
| B | ILE293 |
| B | ASP316 |
| B | MG1369 |
| B | HOH2041 |
| site_id | AC3 |
| Number of Residues | 17 |
| Details | BINDING SITE FOR RESIDUE AME C 1368 |
| Chain | Residue |
| C | PHE19 |
| C | PHE23 |
| C | GLN26 |
| C | MET50 |
| C | SER135 |
| C | LYS161 |
| C | LYS163 |
| C | ASP189 |
| C | ASN191 |
| C | GLU214 |
| C | ASP239 |
| C | LYS263 |
| C | ASP291 |
| C | MET292 |
| C | ILE293 |
| C | MG1369 |
| C | HOH2028 |
| site_id | AC4 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE AME D 1368 |
| Chain | Residue |
| D | PHE19 |
| D | THR21 |
| D | PHE23 |
| D | SER135 |
| D | LYS161 |
| D | LYS163 |
| D | ASP189 |
| D | ASN191 |
| D | GLU214 |
| D | LYS263 |
| D | ASP291 |
| D | MET292 |
| D | ASP316 |
| D | MG1369 |
| D | HOH2030 |
| site_id | AC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MG D 1369 |
| Chain | Residue |
| D | ASP189 |
| D | ASN191 |
| D | GLU214 |
| D | ASP239 |
| D | LYS263 |
| D | AME1368 |
| D | HOH2035 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG A 1370 |
| Chain | Residue |
| A | ASP189 |
| A | GLU214 |
| A | ASP239 |
| A | AME1369 |
| A | HOH2065 |
| site_id | AC7 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MG B 1369 |
| Chain | Residue |
| B | ASP189 |
| B | GLU214 |
| B | ASP239 |
| B | AME1368 |
| B | HOH2044 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE MG C 1369 |
| Chain | Residue |
| C | ASP189 |
| C | ASN191 |
| C | GLU214 |
| C | ASP239 |
| C | GLU240 |
| C | AME1368 |
| C | HOH2031 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor => ECO:0000305|PubMed:14705949, ECO:0000305|PubMed:15134446 |
| Chain | Residue | Details |
| A | LYS163 | |
| B | LYS163 | |
| C | LYS163 | |
| D | LYS163 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000305|PubMed:14705949, ECO:0000305|PubMed:15134446 |
| Chain | Residue | Details |
| A | LYS263 | |
| B | LYS263 | |
| C | LYS263 | |
| D | LYS263 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15134446, ECO:0007744|PDB:1SJB |
| Chain | Residue | Details |
| A | SER135 | |
| C | LYS161 | |
| C | ASN191 | |
| C | ILE293 | |
| D | SER135 | |
| D | LYS161 | |
| D | ASN191 | |
| D | ILE293 | |
| A | LYS161 | |
| A | ASN191 | |
| A | ILE293 | |
| B | SER135 | |
| B | LYS161 | |
| B | ASN191 | |
| B | ILE293 | |
| C | SER135 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:15134446, ECO:0000269|PubMed:23130969, ECO:0000269|Ref.9, ECO:0007744|PDB:1SJA, ECO:0007744|PDB:1SJB, ECO:0007744|PDB:1SJC, ECO:0007744|PDB:4A6G, ECO:0007744|PDB:5FJO, ECO:0007744|PDB:5FJP, ECO:0007744|PDB:5FJR, ECO:0007744|PDB:5FJT, ECO:0007744|PDB:5FJU |
| Chain | Residue | Details |
| A | ASP189 | |
| D | ASP189 | |
| D | GLU214 | |
| D | ASP239 | |
| A | GLU214 | |
| A | ASP239 | |
| B | ASP189 | |
| B | GLU214 | |
| B | ASP239 | |
| C | ASP189 | |
| C | GLU214 | |
| C | ASP239 |
