Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

3RHJ

Crystal structure of the E673A mutant of the C-terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016491	molecular_function	oxidoreductase activity
A	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
B	0016491	molecular_function	oxidoreductase activity
B	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
C	0016491	molecular_function	oxidoreductase activity
C	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
D	0016491	molecular_function	oxidoreductase activity
D	0016620	molecular_function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor

Functional Information from PDB Data

site_id	AC1
Number of Residues	36
Details	BINDING SITE FOR RESIDUE NAP A 101

Chain	Residue
A	VAL570
A	GLY634
A	GLN635
A	PHE648
A	THR649
A	GLY650
A	SER651
A	VAL654
A	HIS657
A	ILE658
A	ALA673
A	ILE571
A	LEU674
A	GLY675
A	CYS707
A	GLU804
A	PHE806
A	LEU834
A	PHE872
A	HOH1095
A	HOH1110
A	HOH1111
A	TRP573
A	HOH1166
A	HOH1257
A	HOH1262
A	HOH1265
A	HOH1276
A	HOH1295
A	HOH1332
A	ASN574
A	LYS597
A	ALA599
A	GLN600
A	SER629
A	GLY630

site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 102

Chain	Residue
A	LYS852
C	ARG554

site_id	AC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE SO4 A 103

Chain	Residue
A	ARG636

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 104

Chain	Residue
A	ARG551
A	ARG554
C	LYS852

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 105

Chain	Residue
A	ASN548
A	HOH1138
B	GLN528

site_id	AC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 106

Chain	Residue
A	ASN738
A	GLU741
A	ARG781
C	HOH1285

site_id	AC7
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 107

Chain	Residue
A	LYS669
A	LEU893
A	ARG894
A	ILE895
A	HOH1144
A	HOH1288
B	GLY881
B	LYS882
B	HOH1001

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL A 108

Chain	Residue
A	TYR575
A	MET578
A	TRP582
A	ASN706
A	CYS707
A	THR866
A	HOH1348

site_id	AC9
Number of Residues	36
Details	BINDING SITE FOR RESIDUE NAP B 101

Chain	Residue
B	HOH1314
B	HOH1320
B	VAL570
B	ILE571
B	TRP573
B	ASN574
B	LYS597
B	ALA599
B	GLN600
B	SER629
B	GLY630
B	GLY634
B	GLN635
B	PHE648
B	THR649
B	GLY650
B	SER651
B	VAL654
B	HIS657
B	ILE658
B	ALA673
B	LEU674
B	GLY675
B	CYS707
B	GLU804
B	PHE806
B	LEU834
B	PHE872
B	HOH1040
B	HOH1060
B	HOH1143
B	HOH1149
B	HOH1176
B	HOH1264
B	HOH1281
B	HOH1297

site_id	BC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 102

Chain	Residue
B	ARG554
D	LYS852
D	HOH1179

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 103

Chain	Residue
B	ARG636
B	PRO641
B	HOH1270
B	HOH1319

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 B 104

Chain	Residue
B	ARG551
B	ARG554
D	TYR848

site_id	BC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 B 105

Chain	Residue
A	GLN528
B	ASN548
B	HOH1095
B	HOH1137
B	HOH1251
B	HOH1253

site_id	BC5
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 B 106

Chain	Residue
B	THR445
B	ASN738
B	GLU741
B	ARG781
B	HOH1141
B	HOH1279

site_id	BC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 B 107

Chain	Residue
A	GLY881
A	LYS882
A	HOH1002
B	LYS669
B	LEU893
B	ARG894
B	ILE895
B	HOH1292
B	HOH1339

site_id	BC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL B 108

Chain	Residue
B	TYR575
B	ASN706
B	ASN864
B	THR866
B	HOH1344

site_id	BC8
Number of Residues	36
Details	BINDING SITE FOR RESIDUE NAP C 101

Chain	Residue
C	VAL570
C	ILE571
C	PRO572
C	TRP573
C	ASN574
C	LYS597
C	ALA599
C	GLN600
C	GLY630
C	GLY634
C	GLN635
C	PHE648
C	THR649
C	GLY650
C	SER651
C	VAL654
C	HIS657
C	ILE658
C	ALA673
C	LEU674
C	GLY675
C	CYS707
C	GLU804
C	PHE806
C	LEU834
C	PHE872
C	HOH1056
C	HOH1065
C	HOH1087
C	HOH1091
C	HOH1132
C	HOH1133
C	HOH1218
C	HOH1312
C	HOH1334
C	HOH1381

site_id	BC9
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 102

Chain	Residue
A	ARG554
C	LYS852

site_id	CC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 103

Chain	Residue
C	ARG636
C	PRO641

site_id	CC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 C 104

Chain	Residue
C	ARG551
C	ARG554

site_id	CC3
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 C 105

Chain	Residue
C	ASN548
C	HOH1233
C	HOH1243
C	HOH1283
C	HOH1390
D	GLN528

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 C 106

Chain	Residue
A	LYS415
C	ASN738
C	ARG781
C	HOH1039
C	HOH1328

site_id	CC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SO4 C 107

Chain	Residue
C	LYS669
C	LEU893
C	ARG894
C	ILE895
C	HOH1072
C	HOH1140
C	HOH1255
D	GLY881
D	LYS882
D	HOH1002

site_id	CC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 C 108

Chain	Residue
A	LEU416
A	THR417
C	LYS415
C	ARG742

site_id	CC7
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL C 109

Chain	Residue
C	TYR575
C	TRP582
C	ASN706
C	CYS707
C	THR866
C	HOH1330

site_id	CC8
Number of Residues	35
Details	BINDING SITE FOR RESIDUE NAP D 101

Chain	Residue
D	VAL570
D	ILE571
D	TRP573
D	ASN574
D	LYS597
D	ALA599
D	GLN600
D	SER629
D	GLY630
D	GLY634
D	GLN635
D	PHE648
D	THR649
D	GLY650
D	SER651
D	VAL654
D	HIS657
D	ILE658
D	ALA673
D	LEU674
D	GLY675
D	CYS707
D	GLU804
D	PHE806
D	LEU834
D	PHE872
D	HOH1035
D	HOH1049
D	HOH1085
D	HOH1135
D	HOH1198
D	HOH1284
D	HOH1298
D	HOH1307
D	HOH1319

site_id	CC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 102

Chain	Residue
B	ASP851
B	LYS852
B	HOH1125
D	ARG554

site_id	DC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 D 103

Chain	Residue
D	ARG636
D	PRO641
D	HOH1338

site_id	DC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 D 104

Chain	Residue
D	ARG551
D	ARG554

site_id	DC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 D 105

Chain	Residue
D	ASN738
D	GLU741
D	ARG781
D	HOH1153

site_id	DC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 D 106

Chain	Residue
C	GLY881
C	LYS882
C	HOH1006
D	LYS669
D	LEU893
D	ARG894
D	ILE895
D	HOH1227
D	HOH1309

site_id	DC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL D 107

Chain	Residue
D	MET578
D	ASN706
D	THR866
D	HOH1351

Functional Information from PROSITE/UniProt

site_id	PS00070
Number of Residues	12
Details	ALDEHYDE_DEHYDR_CYS Aldehyde dehydrogenases cysteine active site. FfNKGENCIAAG

Chain	Residue	Details
A	PHE700-GLY711

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000305\|PubMed:17302434

Chain	Residue	Details
A	ALA673
B	ALA673
C	ALA673
D	ALA673

site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Proton donor => ECO:0000305\|PubMed:17302434, ECO:0000305\|PubMed:21540484, ECO:0007744\|PDB:2O2Q, ECO:0007744\|PDB:3RHO

Chain	Residue	Details
A	CYS707
B	CYS707
C	CYS707
D	CYS707

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:17302434, ECO:0000269\|PubMed:21540484, ECO:0007744\|PDB:2O2Q, ECO:0007744\|PDB:2O2R, ECO:0007744\|PDB:3RHJ, ECO:0007744\|PDB:3RHL, ECO:0007744\|PDB:3RHO, ECO:0007744\|PDB:3RHQ, ECO:0007744\|PDB:3RHR

Chain	Residue	Details
A	LYS597
B	ILE571
B	LYS597
B	GLY630
B	GLY650
C	ILE571
C	LYS597
C	GLY630
C	GLY650
D	ILE571
D	LYS597
D	GLY630
D	GLY650
A	ILE571
A	GLY630
A	GLY650

site_id	SWS_FT_FI4
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269\|PubMed:17302434, ECO:0000269\|PubMed:21540484, ECO:0007744\|PDB:2O2Q, ECO:0007744\|PDB:3RHJ, ECO:0007744\|PDB:3RHL, ECO:0007744\|PDB:3RHO, ECO:0007744\|PDB:3RHQ, ECO:0007744\|PDB:3RHR

Chain	Residue	Details
C	ALA673
C	GLU804
D	ALA673
D	GLU804
A	ALA673
A	GLU804
B	ALA673
B	GLU804

site_id	SWS_FT_FI5
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:21540484, ECO:0007744\|PDB:3RHO

Chain	Residue	Details
A	LYS757
B	LYS757
C	LYS757
D	LYS757

site_id	SWS_FT_FI6
Number of Residues	12
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:O75891

Chain	Residue	Details
B	SER631
B	SER825
C	SER629
C	SER631
C	SER825
D	SER629
D	SER631
D	SER825
A	SER629
A	SER631
A	SER825
B	SER629

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: N6-succinyllysine => ECO:0000250\|UniProtKB:Q8K009

Chain	Residue	Details
A	LYS660
B	LYS660
C	LYS660
D	LYS660

site_id	SWS_FT_FI8
Number of Residues	4
Details	MOD_RES: N6-succinyllysine => ECO:0000250\|UniProtKB:Q8R0Y6

Chain	Residue	Details
A	LYS767
B	LYS767
C	LYS767
D	LYS767

site_id	SWS_FT_FI9
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0000250\|UniProtKB:Q3SY69

Chain	Residue	Details
A	LYS882
B	LYS882
C	LYS882
D	LYS882

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 766

Chain	Residue	Details
A	CYS707

site_id	MCSA2
Number of Residues	1
Details	M-CSA 766

Chain	Residue	Details
B	CYS707

site_id	MCSA3
Number of Residues	1
Details	M-CSA 766

Chain	Residue	Details
C	CYS707

site_id	MCSA4
Number of Residues	1
Details	M-CSA 766

Chain	Residue	Details
D	CYS707

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)