3R8W
Structure of 3-isopropylmalate dehydrogenase isoform 2 from Arabidopsis thaliana at 2.2 angstrom resolution
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000287 | molecular_function | magnesium ion binding |
| A | 0003862 | molecular_function | 3-isopropylmalate dehydrogenase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0009098 | biological_process | L-leucine biosynthetic process |
| A | 0009507 | cellular_component | chloroplast |
| A | 0009536 | cellular_component | plastid |
| A | 0009553 | biological_process | embryo sac development |
| A | 0009555 | biological_process | pollen development |
| A | 0009570 | cellular_component | chloroplast stroma |
| A | 0009941 | cellular_component | chloroplast envelope |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0051287 | molecular_function | NAD binding |
| A | 0070403 | molecular_function | NAD+ binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0003862 | molecular_function | 3-isopropylmalate dehydrogenase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0009098 | biological_process | L-leucine biosynthetic process |
| B | 0009507 | cellular_component | chloroplast |
| B | 0009536 | cellular_component | plastid |
| B | 0009553 | biological_process | embryo sac development |
| B | 0009555 | biological_process | pollen development |
| B | 0009570 | cellular_component | chloroplast stroma |
| B | 0009941 | cellular_component | chloroplast envelope |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0051287 | molecular_function | NAD binding |
| B | 0070403 | molecular_function | NAD+ binding |
| C | 0000287 | molecular_function | magnesium ion binding |
| C | 0003862 | molecular_function | 3-isopropylmalate dehydrogenase activity |
| C | 0005829 | cellular_component | cytosol |
| C | 0009098 | biological_process | L-leucine biosynthetic process |
| C | 0009507 | cellular_component | chloroplast |
| C | 0009536 | cellular_component | plastid |
| C | 0009553 | biological_process | embryo sac development |
| C | 0009555 | biological_process | pollen development |
| C | 0009570 | cellular_component | chloroplast stroma |
| C | 0009941 | cellular_component | chloroplast envelope |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0042803 | molecular_function | protein homodimerization activity |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0051287 | molecular_function | NAD binding |
| C | 0070403 | molecular_function | NAD+ binding |
| D | 0000287 | molecular_function | magnesium ion binding |
| D | 0003862 | molecular_function | 3-isopropylmalate dehydrogenase activity |
| D | 0005829 | cellular_component | cytosol |
| D | 0009098 | biological_process | L-leucine biosynthetic process |
| D | 0009507 | cellular_component | chloroplast |
| D | 0009536 | cellular_component | plastid |
| D | 0009553 | biological_process | embryo sac development |
| D | 0009555 | biological_process | pollen development |
| D | 0009570 | cellular_component | chloroplast stroma |
| D | 0009941 | cellular_component | chloroplast envelope |
| D | 0016491 | molecular_function | oxidoreductase activity |
| D | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| D | 0042803 | molecular_function | protein homodimerization activity |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0051287 | molecular_function | NAD binding |
| D | 0070403 | molecular_function | NAD+ binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE ACT A 368 |
| Chain | Residue |
| A | LYS151 |
| A | PHE161 |
| B | GLU146 |
| site_id | AC2 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE ACT A 369 |
| Chain | Residue |
| A | HIS169 |
| A | ARG173 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT A 370 |
| Chain | Residue |
| A | GLU91 |
| A | ARG98 |
| A | ARG108 |
| A | ARG136 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE ACT A 371 |
| Chain | Residue |
| A | ASP235 |
| A | GLN238 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT A 372 |
| Chain | Residue |
| A | ILE15 |
| A | ASN296 |
| A | ASP337 |
| A | HOH487 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE ACT B 368 |
| Chain | Residue |
| B | HIS169 |
| B | ARG173 |
| site_id | AC7 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE ACT B 369 |
| Chain | Residue |
| B | ASP235 |
| B | GLN238 |
| site_id | AC8 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE ACT B 370 |
| Chain | Residue |
| B | ASP254 |
| B | ILE282 |
| site_id | AC9 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE ACT B 371 |
| Chain | Residue |
| B | ASN296 |
| B | ASP337 |
| site_id | BC1 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE ACT B 372 |
| Chain | Residue |
| B | ARG207 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT B 373 |
| Chain | Residue |
| B | GLU91 |
| B | ARG98 |
| B | ARG108 |
| B | ARG136 |
| site_id | BC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE ACT C 368 |
| Chain | Residue |
| C | ARG98 |
| C | ARG108 |
| C | ARG136 |
| site_id | BC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ACT D 368 |
| Chain | Residue |
| D | GLU91 |
| D | ARG98 |
| D | ARG108 |
| D | ARG136 |
| site_id | BC5 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE ACT D 369 |
| Chain | Residue |
| D | ASP235 |
| D | LYS237 |
| D | GLN238 |
| site_id | BC6 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE ACT D 370 |
| Chain | Residue |
| D | ARG108 |
| D | ASP254 |
Functional Information from PROSITE/UniProt
| site_id | PS00470 |
| Number of Residues | 20 |
| Details | IDH_IMDH Isocitrate and isopropylmalate dehydrogenases signature. NIFGDIlSDeaSmit.GSIGM |
| Chain | Residue | Details |
| A | ASN246-MET265 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27137927, ECO:0007744|PDB:5J33 |
| Chain | Residue | Details |
| A | ILE76 | |
| A | ASN196 | |
| A | ASN227 | |
| C | ILE76 | |
| C | ASN196 | |
| C | ASN227 | |
| C | GLU280 | |
| D | ILE76 | |
| D | ASN196 | |
| D | ASN227 | |
| D | GLU280 | |
| A | GLU280 | |
| B | ILE76 | |
| B | ASN196 | |
| B | ASN227 | |
| B | GLU280 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 16 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27137927, ECO:0007744|PDB:5J32 |
| Chain | Residue | Details |
| A | ARG98 | |
| A | ARG108 | |
| A | ARG136 | |
| A | ASP226 | |
| D | ARG98 | |
| D | ARG108 | |
| D | ARG136 | |
| D | ASP226 | |
| B | ARG98 | |
| B | ARG108 | |
| B | ARG136 | |
| B | ASP226 | |
| C | ARG98 | |
| C | ARG108 | |
| C | ARG136 | |
| C | ASP226 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 8 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:27137927, ECO:0007744|PDB:5J32, ECO:0007744|PDB:5J33, ECO:0007744|PDB:5J34 |
| Chain | Residue | Details |
| A | ASP250 | |
| A | ASP254 | |
| B | ASP250 | |
| B | ASP254 | |
| C | ASP250 | |
| C | ASP254 | |
| D | ASP250 | |
| D | ASP254 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | SITE: Confers substrate specificity => ECO:0000269|PubMed:21697089 |
| Chain | Residue | Details |
| A | LEU95 | |
| B | LEU95 | |
| C | LEU95 | |
| D | LEU95 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | SITE: Important for catalysis => ECO:0000269|PubMed:27137927 |
| Chain | Residue | Details |
| C | TYR143 | |
| C | LYS194 | |
| D | TYR143 | |
| D | LYS194 | |
| A | TYR143 | |
| A | LYS194 | |
| B | TYR143 | |
| B | LYS194 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 8 |
| Details | SITE: Essential for redox regulation => ECO:0000250|UniProtKB:Q9FMT1 |
| Chain | Residue | Details |
| A | CYS190 | |
| A | CYS348 | |
| B | CYS190 | |
| B | CYS348 | |
| C | CYS190 | |
| C | CYS348 | |
| D | CYS190 | |
| D | CYS348 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q9FMT1 |
| Chain | Residue | Details |
| A | SER32 | |
| B | SER32 | |
| C | SER32 | |
| D | SER32 |
