3DV3

MEK1 with PF-04622664 Bound

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 1

Chain	Residue
A	ASN195
A	ASP208
A	HOH9020
A	HOH9078

---

site_id	AC2
Number of Residues	22
Details	BINDING SITE FOR RESIDUE ATP A 9000

Chain	Residue
A	LYS97
A	MET143
A	GLU144
A	MET146
A	SER150
A	GLN153
A	LYS192
A	SER194
A	ASN195
A	LEU197
A	ASP208
A	HOH9011
A	HOH9020
A	HOH9054
A	HOH9069
A	HOH9078
A	HOH9079
A	ALA76
A	GLY77
A	ASN78
A	GLY80
A	ALA95

---

site_id	AC3
Number of Residues	12
Details	BINDING SITE FOR RESIDUE MEK A 9001

Chain	Residue
A	LYS97
A	LEU115
A	ILE141
A	MET143
A	ASP208
A	PHE209
A	GLY210
A	SER212
A	LEU215
A	HOH9008
A	HOH9011
A	HOH9020

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 9003

Chain	Residue
A	LEU63
A	GLN116
A	HIS119
A	GLY131
A	ALA132

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGAGNGGVVFkVshkpsglv..........MARK

Chain	Residue	Details
A	LEU74-LYS97

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ImHrDVKpsNILV

Chain	Residue	Details
A	ILE186-VAL198

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027

Chain	Residue	Details
A	ASP190

---

site_id	SWS_FT_FI2
Number of Residues	5
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000269|PubMed:15543157, ECO:0000269|PubMed:17880056, ECO:0000269|PubMed:18951019, ECO:0000269|PubMed:19019675, ECO:0000269|PubMed:19706763, ECO:0000269|PubMed:21310613

Chain	Residue	Details
A	MET143
A	SER150
A	LYS192
A	ASP208
A	LEU74

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:19161339, ECO:0007744|PDB:3EQH

Chain	Residue	Details
A	LYS97

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:19161339, ECO:0007744|PDB:3EQF

Chain	Residue	Details
A	SER194
A	GLU144

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine; by BRAF and RAF1 => ECO:0000269|PubMed:10409742, ECO:0000269|PubMed:20956560, ECO:0000269|PubMed:29433126, ECO:0000269|PubMed:8131746

Chain	Residue	Details
A	SER218

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphoserine; by BRAF and RAF1 => ECO:0000269|PubMed:20956560, ECO:0000269|PubMed:29433126, ECO:0000269|PubMed:8131746

Chain	Residue	Details
A	SER222

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:18691976

Chain	Residue	Details
A	THR286

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphothreonine; by MAPK1 => ECO:0000250|UniProtKB:Q01986

Chain	Residue	Details
A	THR292

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PAK => ECO:0000269|PubMed:16129686

Chain	Residue	Details
A	SER298

---