2VS5
THE BINDING OF UDP-GALACTOSE BY AN ACTIVE SITE MUTANT OF alpha-1,3 GALACTOSYLTRANSFERASE (alpha3GT)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005975 | biological_process | carbohydrate metabolic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016758 | molecular_function | hexosyltransferase activity |
| B | 0005975 | biological_process | carbohydrate metabolic process |
| B | 0016020 | cellular_component | membrane |
| B | 0016758 | molecular_function | hexosyltransferase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MN A1366 |
| Chain | Residue |
| A | ASP225 |
| A | ASP227 |
| A | GDU1368 |
| A | HOH2348 |
| A | HOH2354 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MN A1367 |
| Chain | Residue |
| A | ASP340 |
| A | HIS342 |
| A | HOH2349 |
| site_id | AC3 |
| Number of Residues | 27 |
| Details | BINDING SITE FOR RESIDUE GDU A1368 |
| Chain | Residue |
| A | ALA135 |
| A | VAL136 |
| A | TYR139 |
| A | TRP195 |
| A | ILE198 |
| A | SER199 |
| A | ARG202 |
| A | ASP225 |
| A | VAL226 |
| A | ASP227 |
| A | HIS280 |
| A | ALA281 |
| A | ALA282 |
| A | TRP314 |
| A | HIS315 |
| A | ASP316 |
| A | GLN317 |
| A | VAL364 |
| A | ARG365 |
| A | MN1366 |
| A | HOH2205 |
| A | HOH2350 |
| A | HOH2351 |
| A | HOH2352 |
| A | HOH2353 |
| A | HOH2354 |
| A | PHE134 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MN B1360 |
| Chain | Residue |
| B | ASP225 |
| B | ASP227 |
| B | GDU1361 |
| B | HOH2364 |
| site_id | AC5 |
| Number of Residues | 24 |
| Details | BINDING SITE FOR RESIDUE GDU B1361 |
| Chain | Residue |
| B | PHE134 |
| B | ALA135 |
| B | VAL136 |
| B | TYR139 |
| B | TRP195 |
| B | ILE198 |
| B | SER199 |
| B | ARG202 |
| B | ASP225 |
| B | VAL226 |
| B | ASP227 |
| B | HIS280 |
| B | ALA281 |
| B | ALA282 |
| B | TRP314 |
| B | HIS315 |
| B | ASP316 |
| B | GLN317 |
| B | MN1360 |
| B | HOH2217 |
| B | HOH2329 |
| B | HOH2365 |
| B | HOH2366 |
| B | HOH2367 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MPD B1362 |
| Chain | Residue |
| B | ARG194 |
| B | ASP197 |
| B | LYS306 |
| B | HOH2167 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Nucleophile => ECO:0000269|PubMed:11179209, ECO:0007744|PDB:1G8O |
| Chain | Residue | Details |
| A | GLN317 | |
| B | GLN317 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11179209, ECO:0000269|PubMed:11592969, ECO:0000269|PubMed:12011052, ECO:0007744|PDB:1G93, ECO:0007744|PDB:1GWV, ECO:0007744|PDB:1GWW, ECO:0007744|PDB:1GX0, ECO:0007744|PDB:1GX4, ECO:0007744|PDB:1K4V, ECO:0007744|PDB:1O7O, ECO:0007744|PDB:1O7Q, ECO:0007744|PDB:1VZT, ECO:0007744|PDB:1VZU, ECO:0007744|PDB:1VZX, ECO:0007744|PDB:2JCK, ECO:0007744|PDB:2VFZ, ECO:0007744|PDB:2VS3, ECO:0007744|PDB:2VS4, ECO:0007744|PDB:2VS5, ECO:0007744|PDB:2VXL, ECO:0007744|PDB:2WGZ |
| Chain | Residue | Details |
| A | PHE134 | |
| B | PHE134 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11179209, ECO:0000269|PubMed:11592969, ECO:0000269|PubMed:12011052, ECO:0007744|PDB:1G8O, ECO:0007744|PDB:1G93, ECO:0007744|PDB:1GWV, ECO:0007744|PDB:1GWW, ECO:0007744|PDB:1GX0, ECO:0007744|PDB:1GX4, ECO:0007744|PDB:1K4V, ECO:0007744|PDB:1O7O, ECO:0007744|PDB:1O7Q, ECO:0007744|PDB:1VZT, ECO:0007744|PDB:1VZU, ECO:0007744|PDB:1VZX, ECO:0007744|PDB:2JCK, ECO:0007744|PDB:2VFZ, ECO:0007744|PDB:2VS3, ECO:0007744|PDB:2VS4, ECO:0007744|PDB:2VS5, ECO:0007744|PDB:2VXL, ECO:0007744|PDB:2WGZ |
| Chain | Residue | Details |
| B | ASP225 | |
| B | ASP227 | |
| A | ASP225 | |
| A | ASP227 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:12011052, ECO:0007744|PDB:1GWV, ECO:0007744|PDB:1GX4, ECO:0007744|PDB:1O7O, ECO:0007744|PDB:1O7Q, ECO:0007744|PDB:1VZX, ECO:0007744|PDB:2VS4, ECO:0007744|PDB:2WGZ |
| Chain | Residue | Details |
| B | GLN247 | |
| B | THR259 | |
| A | GLN247 | |
| A | THR259 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11592969, ECO:0000269|PubMed:12011052, ECO:0007744|PDB:1GWV, ECO:0007744|PDB:1GWW, ECO:0007744|PDB:1GX0, ECO:0007744|PDB:1GX4, ECO:0007744|PDB:1K4V, ECO:0007744|PDB:1O7O, ECO:0007744|PDB:1O7Q, ECO:0007744|PDB:1VZT, ECO:0007744|PDB:1VZU, ECO:0007744|PDB:1VZX, ECO:0007744|PDB:2JCK, ECO:0007744|PDB:2VFZ, ECO:0007744|PDB:2VS3, ECO:0007744|PDB:2VS4, ECO:0007744|PDB:2VS5, ECO:0007744|PDB:2WGZ |
| Chain | Residue | Details |
| A | LYS359 | |
| B | LYS359 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255 |
| Chain | Residue | Details |
| A | ASN293 | |
| B | ASN293 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 6 |
| Details | M-CSA 356 |
| Chain | Residue | Details |
| A | GLN247 | electrostatic stabiliser, hydrogen bond donor |
| A | HIS280 | electrostatic stabiliser, hydrogen bond donor |
| A | TRP314 | electrostatic stabiliser, hydrogen bond donor |
| A | GLN317 | activator, covalently attached, electrostatic stabiliser, nucleofuge, nucleophile |
| A | TRP356 | electrostatic stabiliser, hydrogen bond donor |
| A | ARG365 | electrostatic stabiliser |
| site_id | MCSA2 |
| Number of Residues | 6 |
| Details | M-CSA 356 |
| Chain | Residue | Details |
| B | GLN247 | electrostatic stabiliser, hydrogen bond donor |
| B | HIS280 | electrostatic stabiliser, hydrogen bond donor |
| B | TRP314 | electrostatic stabiliser, hydrogen bond donor |
| B | GLN317 | activator, covalently attached, electrostatic stabiliser, nucleofuge, nucleophile |
| B | TRP356 | electrostatic stabiliser, hydrogen bond donor |
| B | ARG365 | electrostatic stabiliser |
