2V9N
L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT A88F- E192A)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005996 | biological_process | monosaccharide metabolic process |
| A | 0008994 | molecular_function | rhamnulose-1-phosphate aldolase activity |
| A | 0016829 | molecular_function | lyase activity |
| A | 0016830 | molecular_function | carbon-carbon lyase activity |
| A | 0016832 | molecular_function | aldehyde-lyase activity |
| A | 0019299 | biological_process | rhamnose metabolic process |
| A | 0019301 | biological_process | rhamnose catabolic process |
| A | 0019323 | biological_process | pentose catabolic process |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005996 | biological_process | monosaccharide metabolic process |
| B | 0008994 | molecular_function | rhamnulose-1-phosphate aldolase activity |
| B | 0016829 | molecular_function | lyase activity |
| B | 0016830 | molecular_function | carbon-carbon lyase activity |
| B | 0016832 | molecular_function | aldehyde-lyase activity |
| B | 0019299 | biological_process | rhamnose metabolic process |
| B | 0019301 | biological_process | rhamnose catabolic process |
| B | 0019323 | biological_process | pentose catabolic process |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0046872 | molecular_function | metal ion binding |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0005996 | biological_process | monosaccharide metabolic process |
| C | 0008994 | molecular_function | rhamnulose-1-phosphate aldolase activity |
| C | 0016829 | molecular_function | lyase activity |
| C | 0016830 | molecular_function | carbon-carbon lyase activity |
| C | 0016832 | molecular_function | aldehyde-lyase activity |
| C | 0019299 | biological_process | rhamnose metabolic process |
| C | 0019301 | biological_process | rhamnose catabolic process |
| C | 0019323 | biological_process | pentose catabolic process |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0046872 | molecular_function | metal ion binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0005996 | biological_process | monosaccharide metabolic process |
| D | 0008994 | molecular_function | rhamnulose-1-phosphate aldolase activity |
| D | 0016829 | molecular_function | lyase activity |
| D | 0016830 | molecular_function | carbon-carbon lyase activity |
| D | 0016832 | molecular_function | aldehyde-lyase activity |
| D | 0019299 | biological_process | rhamnose metabolic process |
| D | 0019301 | biological_process | rhamnose catabolic process |
| D | 0019323 | biological_process | pentose catabolic process |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN A1275 |
| Chain | Residue |
| A | HIS141 |
| A | HIS143 |
| A | HIS212 |
| A | CIT1276 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN B1275 |
| Chain | Residue |
| B | HIS141 |
| B | HIS143 |
| B | HIS212 |
| B | CIT1276 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN C1275 |
| Chain | Residue |
| C | HIS143 |
| C | HIS212 |
| C | CIT1276 |
| C | HIS141 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE ZN D1275 |
| Chain | Residue |
| D | HIS141 |
| D | HIS143 |
| D | HIS212 |
| D | CIT1276 |
| site_id | AC5 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE CIT A1276 |
| Chain | Residue |
| A | ASN29 |
| A | GLY31 |
| A | ASN32 |
| A | GLU117 |
| A | HIS141 |
| A | HIS143 |
| A | PRO188 |
| A | HIS212 |
| A | ZN1275 |
| A | PO41277 |
| A | HOH2068 |
| A | HOH2292 |
| A | HOH2293 |
| A | HOH2294 |
| C | GLU171 |
| site_id | AC6 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE CIT B1276 |
| Chain | Residue |
| B | ASN29 |
| B | GLY30 |
| B | GLY31 |
| B | ASN32 |
| B | GLU117 |
| B | HIS141 |
| B | HIS143 |
| B | PRO188 |
| B | HIS212 |
| B | ZN1275 |
| B | PO41277 |
| B | HOH2063 |
| B | HOH2177 |
| B | HOH2300 |
| D | GLU171 |
| site_id | AC7 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE CIT C1276 |
| Chain | Residue |
| B | GLU171 |
| C | ASN29 |
| C | GLY30 |
| C | GLY31 |
| C | ASN32 |
| C | GLU117 |
| C | HIS141 |
| C | HIS143 |
| C | PRO188 |
| C | HIS212 |
| C | ZN1275 |
| C | PO41277 |
| C | HOH2063 |
| C | HOH2304 |
| C | HOH2305 |
| site_id | AC8 |
| Number of Residues | 15 |
| Details | BINDING SITE FOR RESIDUE CIT D1276 |
| Chain | Residue |
| A | GLU171 |
| D | ASN29 |
| D | GLY30 |
| D | GLY31 |
| D | ASN32 |
| D | GLU117 |
| D | HIS141 |
| D | HIS143 |
| D | PRO188 |
| D | HIS212 |
| D | ZN1275 |
| D | PO41277 |
| D | HOH2052 |
| D | HOH2161 |
| D | HOH2282 |
| site_id | AC9 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PO4 A1277 |
| Chain | Residue |
| A | ASN29 |
| A | ASN32 |
| A | SER75 |
| A | GLY76 |
| A | THR115 |
| A | SER116 |
| A | CIT1276 |
| A | HOH2068 |
| A | HOH2123 |
| site_id | BC1 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE PO4 B1277 |
| Chain | Residue |
| B | ASN29 |
| B | ASN32 |
| B | SER75 |
| B | GLY76 |
| B | THR115 |
| B | SER116 |
| B | CIT1276 |
| B | HOH2063 |
| B | HOH2065 |
| B | HOH2113 |
| site_id | BC2 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE PO4 C1277 |
| Chain | Residue |
| C | THR115 |
| C | SER116 |
| C | CIT1276 |
| C | HOH2063 |
| C | HOH2125 |
| C | ASN29 |
| C | ASN32 |
| C | SER75 |
| C | GLY76 |
| site_id | BC3 |
| Number of Residues | 10 |
| Details | BINDING SITE FOR RESIDUE PO4 D1277 |
| Chain | Residue |
| D | ASN29 |
| D | ASN32 |
| D | SER75 |
| D | GLY76 |
| D | THR115 |
| D | SER116 |
| D | CIT1276 |
| D | HOH2052 |
| D | HOH2053 |
| D | HOH2098 |
| site_id | BC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE PO4 C1278 |
| Chain | Residue |
| B | HIS50 |
| B | GLN51 |
| B | HOH2087 |
| C | HIS46 |
| C | ASP47 |
| C | HOH2306 |
| C | HOH2307 |
| C | HOH2308 |
| site_id | BC5 |
| Number of Residues | 7 |
| Details | BINDING SITE FOR RESIDUE PO4 D1278 |
| Chain | Residue |
| A | HIS46 |
| A | ASP47 |
| D | HIS50 |
| D | GLN51 |
| D | HOH2283 |
| D | HOH2284 |
| D | HOH2285 |
| site_id | BC6 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PO4 C1279 |
| Chain | Residue |
| C | THR158 |
| C | GLU200 |
| C | LYS203 |
| C | HIS204 |
| C | HOH2309 |
| site_id | BC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PO4 D1279 |
| Chain | Residue |
| D | THR158 |
| D | GLU200 |
| D | HIS204 |
| D | HOH2286 |
| site_id | BC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE PO4 A1278 |
| Chain | Residue |
| A | THR158 |
| A | GLU200 |
| A | LYS203 |
| A | HIS204 |
| A | HOH2295 |
| A | HOH2296 |
| site_id | BC9 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PO4 B1278 |
| Chain | Residue |
| B | THR158 |
| B | GLU200 |
| B | LYS203 |
| B | HIS204 |
| B | HOH2301 |
| site_id | CC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PGO A1279 |
| Chain | Residue |
| A | ARG80 |
| A | GLN83 |
| A | HOH2297 |
| C | HOH2013 |
| site_id | CC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PGO B1279 |
| Chain | Residue |
| B | ARG80 |
| B | GLN83 |
| B | HOH2302 |
| D | HOH2012 |
| D | HOH2032 |
| site_id | CC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PGO C1280 |
| Chain | Residue |
| B | HOH2014 |
| B | HOH2038 |
| C | ARG80 |
| C | GLN83 |
| C | HOH2310 |
| site_id | CC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PGO D1280 |
| Chain | Residue |
| A | HOH2014 |
| A | HOH2037 |
| D | ARG80 |
| D | GLN83 |
| site_id | CC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE PGO A1280 |
| Chain | Residue |
| A | TYR242 |
| A | HOH2298 |
| A | HOH2299 |
| C | MET244 |
| site_id | CC6 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL A1281 |
| Chain | Residue |
| A | THR190 |
| A | ASP191 |
| A | ALA192 |
| B | ARG262 |
| C | THR190 |
| C | ASP191 |
| C | ALA192 |
| C | GOL1281 |
| site_id | CC7 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL B1280 |
| Chain | Residue |
| A | ARG262 |
| B | THR190 |
| B | ASP191 |
| B | ALA192 |
| B | HOH2303 |
| D | THR190 |
| D | ASP191 |
| D | ALA192 |
| D | GOL1281 |
| site_id | CC8 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL C1281 |
| Chain | Residue |
| A | THR190 |
| A | ASP191 |
| A | ALA192 |
| A | GOL1281 |
| B | ARG262 |
| C | THR190 |
| C | ASP191 |
| C | HOH2311 |
| C | HOH2312 |
| site_id | CC9 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL D1281 |
| Chain | Residue |
| A | ARG262 |
| B | THR190 |
| B | ASP191 |
| B | GOL1280 |
| D | THR190 |
| D | ASP191 |
| D | ARG262 |
| D | HOH2288 |
| D | HOH2289 |
| site_id | DC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PGO B1281 |
| Chain | Residue |
| B | MET244 |
| B | HOH2304 |
| B | HOH2305 |
| C | LEU154 |
| C | TYR242 |
| site_id | DC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PGO D1282 |
| Chain | Residue |
| A | MET244 |
| A | HOH2260 |
| D | VAL153 |
| D | LEU154 |
| D | PGO1283 |
| site_id | DC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE PGO D1283 |
| Chain | Residue |
| D | VAL160 |
| D | GLN164 |
| D | PGO1282 |
| D | HOH2290 |
| D | HOH2291 |
| site_id | DC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE GOL D1284 |
| Chain | Residue |
| A | ASP222 |
| A | GLU223 |
| D | GLY76 |
| D | PHE78 |
| D | HOH2133 |
| D | HOH2292 |
| D | HOH2293 |
| D | HOH2294 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: ACT_SITE => ECO:0000269|PubMed:11976494, ECO:0000269|PubMed:12962479 |
| Chain | Residue | Details |
| A | GLU117 | |
| B | GLU117 | |
| C | GLU117 | |
| D | GLU117 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:11976494, ECO:0000269|PubMed:12962479 |
| Chain | Residue | Details |
| A | HIS141 | |
| A | HIS143 | |
| A | HIS212 | |
| B | HIS141 | |
| B | HIS143 | |
| B | HIS212 | |
| C | HIS141 | |
| C | HIS143 | |
| C | HIS212 | |
| D | HIS141 | |
| D | HIS143 | |
| D | HIS212 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 5 |
| Details | M-CSA 645 |
| Chain | Residue | Details |
| A | GLU117 | proton acceptor, proton donor |
| A | HIS141 | metal ligand |
| A | HIS143 | metal ligand |
| A | GLU171 | proton donor |
| A | HIS212 | metal ligand |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 645 |
| Chain | Residue | Details |
| B | GLU117 | proton acceptor, proton donor |
| B | HIS141 | metal ligand |
| B | HIS143 | metal ligand |
| B | GLU171 | proton donor |
| B | HIS212 | metal ligand |
| site_id | MCSA3 |
| Number of Residues | 5 |
| Details | M-CSA 645 |
| Chain | Residue | Details |
| C | GLU117 | proton acceptor, proton donor |
| C | HIS141 | metal ligand |
| C | HIS143 | metal ligand |
| C | GLU171 | proton donor |
| C | HIS212 | metal ligand |
| site_id | MCSA4 |
| Number of Residues | 5 |
| Details | M-CSA 645 |
| Chain | Residue | Details |
| D | GLU117 | proton acceptor, proton donor |
| D | HIS141 | metal ligand |
| D | HIS143 | metal ligand |
| D | GLU171 | proton donor |
| D | HIS212 | metal ligand |
