Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2QFW

Crystal structure of Saccharomyces cerevesiae mitochondrial NADP(+)-dependent isocitrate dehydrogenase in complex with isocitrate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0004450	molecular_function	isocitrate dehydrogenase (NADP+) activity
A	0005515	molecular_function	protein binding
A	0005739	cellular_component	mitochondrion
A	0006097	biological_process	glyoxylate cycle
A	0006099	biological_process	tricarboxylic acid cycle
A	0006102	biological_process	isocitrate metabolic process
A	0006537	biological_process	glutamate biosynthetic process
A	0006739	biological_process	NADP metabolic process
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0042645	cellular_component	mitochondrial nucleoid
A	0046872	molecular_function	metal ion binding
A	0051287	molecular_function	NAD binding
B	0000287	molecular_function	magnesium ion binding
B	0004450	molecular_function	isocitrate dehydrogenase (NADP+) activity
B	0005515	molecular_function	protein binding
B	0005739	cellular_component	mitochondrion
B	0006097	biological_process	glyoxylate cycle
B	0006099	biological_process	tricarboxylic acid cycle
B	0006102	biological_process	isocitrate metabolic process
B	0006537	biological_process	glutamate biosynthetic process
B	0006739	biological_process	NADP metabolic process
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0042645	cellular_component	mitochondrial nucleoid
B	0046872	molecular_function	metal ion binding
B	0051287	molecular_function	NAD binding
C	0000287	molecular_function	magnesium ion binding
C	0004450	molecular_function	isocitrate dehydrogenase (NADP+) activity
C	0005515	molecular_function	protein binding
C	0005739	cellular_component	mitochondrion
C	0006097	biological_process	glyoxylate cycle
C	0006099	biological_process	tricarboxylic acid cycle
C	0006102	biological_process	isocitrate metabolic process
C	0006537	biological_process	glutamate biosynthetic process
C	0006739	biological_process	NADP metabolic process
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0042645	cellular_component	mitochondrial nucleoid
C	0046872	molecular_function	metal ion binding
C	0051287	molecular_function	NAD binding
D	0000287	molecular_function	magnesium ion binding
D	0004450	molecular_function	isocitrate dehydrogenase (NADP+) activity
D	0005515	molecular_function	protein binding
D	0005739	cellular_component	mitochondrion
D	0006097	biological_process	glyoxylate cycle
D	0006099	biological_process	tricarboxylic acid cycle
D	0006102	biological_process	isocitrate metabolic process
D	0006537	biological_process	glutamate biosynthetic process
D	0006739	biological_process	NADP metabolic process
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0042645	cellular_component	mitochondrial nucleoid
D	0046872	molecular_function	metal ion binding
D	0051287	molecular_function	NAD binding
E	0000287	molecular_function	magnesium ion binding
E	0004450	molecular_function	isocitrate dehydrogenase (NADP+) activity
E	0005515	molecular_function	protein binding
E	0005739	cellular_component	mitochondrion
E	0006097	biological_process	glyoxylate cycle
E	0006099	biological_process	tricarboxylic acid cycle
E	0006102	biological_process	isocitrate metabolic process
E	0006537	biological_process	glutamate biosynthetic process
E	0006739	biological_process	NADP metabolic process
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0042645	cellular_component	mitochondrial nucleoid
E	0046872	molecular_function	metal ion binding
E	0051287	molecular_function	NAD binding
F	0000287	molecular_function	magnesium ion binding
F	0004450	molecular_function	isocitrate dehydrogenase (NADP+) activity
F	0005515	molecular_function	protein binding
F	0005739	cellular_component	mitochondrion
F	0006097	biological_process	glyoxylate cycle
F	0006099	biological_process	tricarboxylic acid cycle
F	0006102	biological_process	isocitrate metabolic process
F	0006537	biological_process	glutamate biosynthetic process
F	0006739	biological_process	NADP metabolic process
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0042645	cellular_component	mitochondrial nucleoid
F	0046872	molecular_function	metal ion binding
F	0051287	molecular_function	NAD binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE ICT A 2101

Chain	Residue
A	THR78
B	ASP254
A	SER95
A	ASN97
A	ARG101
A	ARG110
A	ARG133
A	TYR140
A	ASP277
B	LYS214

site_id	AC2
Number of Residues	11
Details	BINDING SITE FOR RESIDUE ICT B 2102

Chain	Residue
A	LYS214
A	ASP254
B	THR78
B	SER95
B	ASN97
B	ARG101
B	ARG110
B	ARG133
B	TYR140
B	ASP277
B	HOH2305

site_id	AC3
Number of Residues	12
Details	BINDING SITE FOR RESIDUE ICT C 2103

Chain	Residue
C	THR78
C	SER95
C	ASN97
C	ARG101
C	ARG110
C	ARG133
C	TYR140
C	ASP277
C	HOH2131
D	LYS214
D	ILE217
D	ASP254

site_id	AC4
Number of Residues	10
Details	BINDING SITE FOR RESIDUE ICT D 2104

Chain	Residue
C	HOH2195
D	SER95
D	ASN97
D	ARG101
D	ARG110
D	ARG133
D	ASP277
D	HOH2135
D	HOH2139
D	HOH2254

site_id	AC5
Number of Residues	12
Details	BINDING SITE FOR RESIDUE ICT E 2105

Chain	Residue
E	THR78
E	SER95
E	ASN97
E	ARG101
E	ARG110
E	ARG133
E	TYR140
E	ASP277
E	HOH2108
F	LYS214
F	ASP254
F	HOH2141

site_id	AC6
Number of Residues	10
Details	BINDING SITE FOR RESIDUE ICT F 2106

Chain	Residue
E	ILE217
E	ASP254
F	THR78
F	SER95
F	ASN97
F	ARG101
F	ARG110
F	ARG133
F	ASP277
F	HOH2126

Functional Information from PROSITE/UniProt

site_id	PS00470
Number of Residues	20
Details	IDH_IMDH Isocitrate and isopropylmalate dehydrogenases signature. NYDGDVqSDivAqgf.GSLGL

Chain	Residue	Details
A	ASN273-LEU292

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	66
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
B	THR78
B	ARG83
B	SER95
B	ARG110
B	ARG133
B	ASP254
B	LYS262
B	ASP277
B	GLY312
B	ASN330
C	THR76
C	THR78
C	ARG83
C	SER95
C	ARG110
C	ARG133
C	ASP254
C	LYS262
C	ASP277
C	GLY312
C	ASN330
D	THR76
D	THR78
D	ARG83
D	SER95
D	ARG110
D	ARG133
D	ASP254
D	LYS262
D	ASP277
D	GLY312
D	ASN330
E	THR76
E	THR78
E	ARG83
E	SER95
E	ARG110
E	ARG133
E	ASP254
E	LYS262
E	ASP277
E	GLY312
E	ASN330
F	THR76
F	THR78
F	ARG83
F	SER95
F	ARG110
F	ARG133
F	ASP254
F	LYS262
F	ASP277
F	GLY312
F	ASN330
A	THR76
A	THR78
A	ARG83
A	SER95
A	ARG110
A	ARG133
A	ASP254
A	LYS262
A	ASP277
A	GLY312
A	ASN330
B	THR76

site_id	SWS_FT_FI2
Number of Residues	12
Details	SITE: Critical for catalysis => ECO:0000250

Chain	Residue	Details
A	TYR140
A	LYS214
B	TYR140
B	LYS214
C	TYR140
C	LYS214
D	TYR140
D	LYS214
E	TYR140
E	LYS214
F	TYR140
F	LYS214

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)