Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

2NQP

Crystal structure of pseudoudirinde synthase TruA in complex with leucyl tRNA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000049	molecular_function	tRNA binding
A	0001522	biological_process	pseudouridine synthesis
A	0003723	molecular_function	RNA binding
A	0008033	biological_process	tRNA processing
A	0009451	biological_process	RNA modification
A	0009982	molecular_function	pseudouridine synthase activity
A	0016853	molecular_function	isomerase activity
A	0031119	biological_process	tRNA pseudouridine synthesis
A	0034470	biological_process	obsolete ncRNA processing
A	0042803	molecular_function	protein homodimerization activity
A	0106029	molecular_function	tRNA pseudouridine synthase activity
A	0140098	molecular_function	catalytic activity, acting on RNA
A	0160147	molecular_function	tRNA pseudouridine(38-40) synthase activity
B	0000049	molecular_function	tRNA binding
B	0001522	biological_process	pseudouridine synthesis
B	0003723	molecular_function	RNA binding
B	0008033	biological_process	tRNA processing
B	0009451	biological_process	RNA modification
B	0009982	molecular_function	pseudouridine synthase activity
B	0016853	molecular_function	isomerase activity
B	0031119	biological_process	tRNA pseudouridine synthesis
B	0034470	biological_process	obsolete ncRNA processing
B	0042803	molecular_function	protein homodimerization activity
B	0106029	molecular_function	tRNA pseudouridine synthase activity
B	0140098	molecular_function	catalytic activity, acting on RNA
B	0160147	molecular_function	tRNA pseudouridine(38-40) synthase activity
C	0000049	molecular_function	tRNA binding
C	0001522	biological_process	pseudouridine synthesis
C	0003723	molecular_function	RNA binding
C	0008033	biological_process	tRNA processing
C	0009451	biological_process	RNA modification
C	0009982	molecular_function	pseudouridine synthase activity
C	0016853	molecular_function	isomerase activity
C	0031119	biological_process	tRNA pseudouridine synthesis
C	0034470	biological_process	obsolete ncRNA processing
C	0042803	molecular_function	protein homodimerization activity
C	0106029	molecular_function	tRNA pseudouridine synthase activity
C	0140098	molecular_function	catalytic activity, acting on RNA
C	0160147	molecular_function	tRNA pseudouridine(38-40) synthase activity
D	0000049	molecular_function	tRNA binding
D	0001522	biological_process	pseudouridine synthesis
D	0003723	molecular_function	RNA binding
D	0008033	biological_process	tRNA processing
D	0009451	biological_process	RNA modification
D	0009982	molecular_function	pseudouridine synthase activity
D	0016853	molecular_function	isomerase activity
D	0031119	biological_process	tRNA pseudouridine synthesis
D	0034470	biological_process	obsolete ncRNA processing
D	0042803	molecular_function	protein homodimerization activity
D	0106029	molecular_function	tRNA pseudouridine synthase activity
D	0140098	molecular_function	catalytic activity, acting on RNA
D	0160147	molecular_function	tRNA pseudouridine(38-40) synthase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	BINDING SITE FOR RESIDUE K F 77

Chain	Residue
F	U60
F	C62

site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE K F 78

Chain	Residue
F	G49
F	G59

site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE K F 79

Chain	Residue
F	U54
F	U55

site_id	AC4
Number of Residues	1
Details	BINDING SITE FOR RESIDUE K C 271

Chain	Residue
C	ALA89

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE K F 80

Chain	Residue
F	K81
F	U20
F	A20

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE K F 81

Chain	Residue
F	U20
F	U48
F	G59
F	U60
F	K80

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Nucleophile => ECO:0000269\|PubMed:10588695, ECO:0000269\|PubMed:9425056

Chain	Residue	Details
A	ASP60
B	ASP60
C	ASP60
D	ASP60

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:17466622

Chain	Residue	Details
A	TYR118
B	TYR118
C	TYR118
D	TYR118

site_id	SWS_FT_FI3
Number of Residues	4
Details	SITE: Interaction with tRNA; Important for base-flipping => ECO:0000269\|PubMed:17466622

Chain	Residue	Details
A	ARG58
B	ARG58
C	ARG58
D	ARG58

site_id	SWS_FT_FI4
Number of Residues	16
Details	SITE: Interaction with tRNA => ECO:0000269\|PubMed:17466622

Chain	Residue	Details
A	ARG78
A	ARG110
A	ARG126
A	PHE139
B	ARG78
B	ARG110
B	ARG126
B	PHE139
C	ARG78
C	ARG110
C	ARG126
C	PHE139
D	ARG78
D	ARG110
D	ARG126
D	PHE139

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 493

Chain	Residue	Details
A	ARG58	electrostatic stabiliser
A	ASP60	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
A	ARG205	electrostatic stabiliser

site_id	MCSA2
Number of Residues	3
Details	M-CSA 493

Chain	Residue	Details
B	ARG58	electrostatic stabiliser
B	ASP60	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
B	ARG205	electrostatic stabiliser

site_id	MCSA3
Number of Residues	3
Details	M-CSA 493

Chain	Residue	Details
C	ARG58	electrostatic stabiliser
C	ASP60	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
C	ARG205	electrostatic stabiliser

site_id	MCSA4
Number of Residues	3
Details	M-CSA 493

Chain	Residue	Details
D	ARG58	electrostatic stabiliser
D	ASP60	covalently attached, nucleofuge, nucleophile, proton acceptor, proton donor
D	ARG205	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)