1WL4

Human cytosolic acetoacetyl-CoA thiolase complexed with CoA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003985	molecular_function	acetyl-CoA C-acetyltransferase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006629	biological_process	lipid metabolic process
A	0006631	biological_process	fatty acid metabolic process
A	0006635	biological_process	fatty acid beta-oxidation
A	0016746	molecular_function	acyltransferase activity
A	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
A	0070062	cellular_component	extracellular exosome

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SO4 A 3001

Chain	Residue
A	SER12
A	HOH3118
A	ALA13
A	ARG44
A	LEU277
A	ASN338
A	HIS368
A	HOH3022
A	HOH3060
A	HOH3090

---

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 3002

Chain	Residue
A	TYR81
A	HIS156
A	GLU289
A	PRO290
A	SER291
A	HOH3207
A	HOH3279
A	HOH3483
A	HOH3502

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 3003

Chain	Residue
A	GLU215
A	VAL216
A	LYS217
A	THR218
A	HOH3408
A	HOH3440
A	HOH3498

---

site_id	AC4
Number of Residues	28
Details	BINDING SITE FOR RESIDUE COA A 1001

Chain	Residue
A	LYS136
A	LEU151
A	HIS159
A	GLN186
A	ARG223
A	SER226
A	MET231
A	TYR237
A	PRO248
A	ALA249
A	SER252
A	GLY253
A	MET293
A	PHE324
A	GOL2001
A	HOH3024
A	HOH3041
A	HOH3050
A	HOH3053
A	HOH3062
A	HOH3068
A	HOH3087
A	HOH3164
A	HOH3225
A	HOH3273
A	HOH3278
A	HOH3351
A	HOH3368

---

site_id	AC5
Number of Residues	8
Details	BINDING SITE FOR RESIDUE GOL A 2001

Chain	Residue
A	ARG223
A	HIS224
A	GLY225
A	ASN227
A	ALA230
A	COA1001
A	HOH3041
A	HOH3465

---

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE GOL A 2002

Chain	Residue
A	ASN187
A	GLU190
A	ASN191
A	ASP270
A	LYS271
A	HOH3402
A	HOH3495
A	HOH3507
A	HOH3546

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL A 2003

Chain	Residue
A	VAL75
A	GLY78
A	ILE79
A	PRO80
A	LYS170

---

Functional Information from PROSITE/UniProt

site_id	PS00099
Number of Residues	14
Details	THIOLASE_3 Thiolases active site. GVAALCIGgGmGiA

Chain	Residue	Details
A	GLY378-ALA391

---

site_id	PS00737
Number of Residues	17
Details	THIOLASE_2 Thiolases signature 2. NieGGaIAlGHPlGaSG

Chain	Residue	Details
A	ASN343-GLY359

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Acyl-thioester intermediate => ECO:0000305|PubMed:15733928

Chain	Residue	Details
A	CSO92

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton donor/acceptor => ECO:0000305|PubMed:15733928

Chain	Residue	Details
A	CYS383

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:15733928, ECO:0007744|PDB:1WL4

Chain	Residue	Details
A	ARG223
A	SER226
A	SER252

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	SITE: Increases nucleophilicity of active site Cys => ECO:0000250|UniProtKB:P42765

Chain	Residue	Details
A	HIS353

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378

Chain	Residue	Details
A	MET1

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
A	LYS200
A	LYS233
A	LYS235

---

Catalytic Information from CSA

site_id	CSA1
Number of Residues	3
Details	Annotated By Reference To The Literature 1afw

Chain	Residue	Details
A	HIS353
A	CYS383
A	GLY385

---

site_id	CSA2
Number of Residues	2
Details	Annotated By Reference To The Literature 1afw

Chain	Residue	Details
A	HIS353
A	CYS383

---