1UXK
Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003824 | molecular_function | catalytic activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006099 | biological_process | tricarboxylic acid cycle |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| A | 0019752 | biological_process | carboxylic acid metabolic process |
| A | 0030060 | molecular_function | L-malate dehydrogenase activity |
| C | 0003824 | molecular_function | catalytic activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0006099 | biological_process | tricarboxylic acid cycle |
| C | 0016491 | molecular_function | oxidoreductase activity |
| C | 0016616 | molecular_function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
| C | 0019752 | biological_process | carboxylic acid metabolic process |
| C | 0030060 | molecular_function | L-malate dehydrogenase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CD A 1308 |
| Chain | Residue |
| A | ASP200 |
| A | ASP243 |
| A | GLU277 |
| A | HOH2280 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CD A 1309 |
| Chain | Residue |
| A | GLU195 |
| A | GLU281 |
| A | HOH2206 |
| C | GLU281 |
| C | CL1312 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CD A 1310 |
| Chain | Residue |
| A | GLU159 |
| A | HOH2168 |
| A | HOH2170 |
| C | GLU159 |
| C | HOH2219 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CD A 1311 |
| Chain | Residue |
| A | GLU178 |
| A | CL1313 |
| A | HOH2077 |
| A | HOH2142 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CD A 1312 |
| Chain | Residue |
| A | ASP148 |
| A | HIS175 |
| A | HOH2162 |
| A | HOH2185 |
| A | HOH2314 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CL A 1313 |
| Chain | Residue |
| A | ASP123 |
| A | ALA124 |
| A | THR302 |
| A | CD1311 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA A 1314 |
| Chain | Residue |
| A | HIS19 |
| A | HIS19 |
| A | HOH2008 |
| A | HOH2008 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE NA A 1315 |
| Chain | Residue |
| A | TYR226 |
| A | HOH2007 |
| A | HOH2007 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA A 1316 |
| Chain | Residue |
| A | ARG2 |
| A | LYS3 |
| A | LEU26 |
| C | HOH2258 |
| site_id | BC1 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CD A 1317 |
| Chain | Residue |
| A | HOH2052 |
| A | HOH2054 |
| A | HOH2247 |
| A | HOH2250 |
| A | HOH2251 |
| C | HOH2176 |
| site_id | BC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE NA A 1318 |
| Chain | Residue |
| A | HOH2078 |
| A | HOH2079 |
| A | HOH2184 |
| A | HOH2188 |
| site_id | BC3 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE NA A 1319 |
| Chain | Residue |
| C | TYR226 |
| C | TYR226 |
| C | HOH2250 |
| C | HOH2250 |
| C | HOH2251 |
| C | HOH2251 |
| site_id | BC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE CD C 1310 |
| Chain | Residue |
| C | ASP148 |
| C | HIS175 |
| C | HOH2153 |
| C | HOH2156 |
| C | HOH2159 |
| site_id | BC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CD C 1311 |
| Chain | Residue |
| C | GLU178 |
| C | HOH2136 |
| C | HOH2139 |
| C | HOH2190 |
| site_id | BC6 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE CL C 1312 |
| Chain | Residue |
| A | GLU281 |
| A | CD1309 |
| A | HOH2204 |
| C | GLU281 |
| site_id | BC7 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CD C 1313 |
| Chain | Residue |
| A | HOH2178 |
| C | HOH2047 |
| C | HOH2048 |
| C | HOH2050 |
| C | HOH2253 |
| C | HOH2254 |
| site_id | BC8 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE CD C 1314 |
| Chain | Residue |
| C | HOH2129 |
| C | HOH2204 |
| C | HOH2207 |
| C | HOH2270 |
| C | HOH2289 |
| C | HOH2290 |
| site_id | BC9 |
| Number of Residues | 1 |
| Details | BINDING SITE FOR RESIDUE NA C 1315 |
| Chain | Residue |
| A | ASP243 |
| site_id | CC1 |
| Number of Residues | 32 |
| Details | BINDING SITE FOR RESIDUE NAD A 1307 |
| Chain | Residue |
| A | HIS175 |
| A | PRO229 |
| A | HOH2075 |
| A | HOH2244 |
| A | HOH2312 |
| A | HOH2313 |
| A | HOH2314 |
| A | HOH2315 |
| A | HOH2316 |
| A | HOH2317 |
| A | HOH2318 |
| A | HOH2319 |
| A | HOH2320 |
| A | GLY9 |
| A | GLY11 |
| A | PHE12 |
| A | VAL13 |
| A | ASP33 |
| A | ILE34 |
| A | VAL35 |
| A | TYR65 |
| A | THR77 |
| A | SER78 |
| A | GLY79 |
| A | ALA80 |
| A | ILE98 |
| A | CYS102 |
| A | VAL118 |
| A | ASN119 |
| A | ASN120 |
| A | GLN143 |
| A | LEU147 |
| site_id | CC2 |
| Number of Residues | 28 |
| Details | BINDING SITE FOR RESIDUE NAD C 1309 |
| Chain | Residue |
| C | GLY9 |
| C | GLY11 |
| C | PHE12 |
| C | VAL13 |
| C | ASP33 |
| C | ILE34 |
| C | VAL35 |
| C | TYR65 |
| C | THR77 |
| C | SER78 |
| C | GLY79 |
| C | ALA80 |
| C | ILE98 |
| C | CYS102 |
| C | VAL118 |
| C | ASN120 |
| C | GLN143 |
| C | LEU147 |
| C | HIS175 |
| C | PRO229 |
| C | HOH2069 |
| C | HOH2153 |
| C | HOH2246 |
| C | HOH2247 |
| C | HOH2319 |
| C | HOH2320 |
| C | HOH2321 |
| C | HOH2322 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000250|UniProtKB:P61889, ECO:0000255|HAMAP-Rule:MF_00487 |
| Chain | Residue | Details |
| A | HIS175 | |
| C | HIS175 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00487, ECO:0000269|PubMed:12054817, ECO:0000269|PubMed:14636605 |
| Chain | Residue | Details |
| A | VAL118 | |
| C | GLY9 | |
| C | ASP33 | |
| C | VAL118 | |
| A | GLY9 | |
| A | ASP33 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 10 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P61889, ECO:0000255|HAMAP-Rule:MF_00487 |
| Chain | Residue | Details |
| A | ASN95 | |
| A | ASN120 | |
| A | ARG151 | |
| C | ARG82 | |
| C | ARG88 | |
| C | ASN95 | |
| C | ASN120 | |
| C | ARG151 | |
| A | ARG82 | |
| A | ARG88 |
