1PUE

PU.1 ETS DOMAIN-DNA COMPLEX

Functional Information from GO Data

Chain	GOid	namespace	contents
E	0003700	molecular_function	DNA-binding transcription factor activity
E	0006355	biological_process	regulation of DNA-templated transcription
E	0006357	biological_process	regulation of transcription by RNA polymerase II
E	0043565	molecular_function	sequence-specific DNA binding
F	0003700	molecular_function	DNA-binding transcription factor activity
F	0006355	biological_process	regulation of DNA-templated transcription
F	0006357	biological_process	regulation of transcription by RNA polymerase II
F	0043565	molecular_function	sequence-specific DNA binding

Functional Information from PROSITE/UniProt

site_id	PS00345
Number of Residues	9
Details	ETS_DOMAIN_1 Ets-domain signature 1. LYQFLLDLL

Chain	Residue	Details
E	LEU174-LEU182

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site_id	PS00346
Number of Residues	16
Details	ETS_DOMAIN_2 Ets-domain signature 2. RkkMtYEkMARALRnY

Chain	Residue	Details
E	ARG222-TYR237

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	166
Details	DNA_BIND: ETS => ECO:0000255|PROSITE-ProRule:PRU00237

Chain	Residue	Details
E	ILE172-SER255
F	ILE172-SER255

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site_id	SWS_FT_FI2
Number of Residues	2
Details	BINDING: forms a salt bridge with the phosphate backbone of the opposite strand downstream of the GGAA core sequence => ECO:0000269|PubMed:8602247

Chain	Residue	Details
E	LYS219
F	LYS219

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site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: contacts bases in the GGAA sequence in the major groove => ECO:0000269|PubMed:8602247, ECO:0007744|PDB:1PUE

Chain	Residue	Details
E	ARG232
E	ARG235
F	ARG232
F	ARG235

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site_id	SWS_FT_FI4
Number of Residues	2
Details	BINDING: contacts the phosphate backbone of the GGAA sequence in the minor groove upstream => ECO:0000269|PubMed:8602247

Chain	Residue	Details
E	LYS245
F	LYS245

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