1OHS

CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANT Y14F/D38N FROM PSEUDOMONAS TESTOSTERONI COMPLEXED WITH ANDROSTANEDIONE

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004769	molecular_function	steroid delta-isomerase activity
A	0008202	biological_process	steroid metabolic process
A	0016853	molecular_function	isomerase activity
B	0004769	molecular_function	steroid delta-isomerase activity
B	0008202	biological_process	steroid metabolic process
B	0016853	molecular_function	isomerase activity
C	0004769	molecular_function	steroid delta-isomerase activity
C	0008202	biological_process	steroid metabolic process
C	0016853	molecular_function	isomerase activity
D	0004769	molecular_function	steroid delta-isomerase activity
D	0008202	biological_process	steroid metabolic process
D	0016853	molecular_function	isomerase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE 5SD A1126

Chain	Residue
A	PHE14
A	LEU18
A	ASN38
A	PHE82
A	PHE86
A	VAL95
A	ASP99
A	MET112
A	PHE116

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site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE 5SD B1326

Chain	Residue
B	PHE214
B	LEU218
B	ASN238
B	PHE254
B	PHE282
B	PHE286
B	ASP299
B	MET312
B	PHE316

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site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE 5SD C1126

Chain	Residue
C	PHE14
C	LEU18
C	ASN38
C	PHE82
C	PHE86
C	VAL95
C	ASP99

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton donor

Chain	Residue	Details
A	PHE14
B	PHE214
C	PHE14
D	PHE214

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site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000269|PubMed:3480517

Chain	Residue	Details
A	ASN38
B	ASN238
C	ASN38
D	ASN238

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site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING:

Chain	Residue	Details
A	ASP99
B	ASP299
C	ASP99
D	ASP299

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