1M1O
Crystal structure of biosynthetic thiolase, C89A mutant, complexed with acetoacetyl-CoA
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006635 | biological_process | fatty acid beta-oxidation |
| A | 0016746 | molecular_function | acyltransferase activity |
| A | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| A | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| B | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006635 | biological_process | fatty acid beta-oxidation |
| B | 0016746 | molecular_function | acyltransferase activity |
| B | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| B | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| C | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006635 | biological_process | fatty acid beta-oxidation |
| C | 0016746 | molecular_function | acyltransferase activity |
| C | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| C | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
| D | 0003985 | molecular_function | acetyl-CoA C-acetyltransferase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006635 | biological_process | fatty acid beta-oxidation |
| D | 0016746 | molecular_function | acyltransferase activity |
| D | 0016747 | molecular_function | acyltransferase activity, transferring groups other than amino-acyl groups |
| D | 0042619 | biological_process | poly-hydroxybutyrate biosynthetic process |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 A 9720 |
| Chain | Residue |
| A | SER260 |
| A | ARG266 |
| site_id | AC2 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE SO4 A 9722 |
| Chain | Residue |
| A | LYS298 |
| A | ARG302 |
| A | HOH474 |
| A | HOH9950 |
| B | GLY106 |
| site_id | AC3 |
| Number of Residues | 29 |
| Details | BINDING SITE FOR RESIDUE CAA A 1393 |
| Chain | Residue |
| A | GLY147 |
| A | LEU148 |
| A | HIS156 |
| A | MET157 |
| A | GLN183 |
| A | ARG220 |
| A | SER227 |
| A | MET228 |
| A | GLY244 |
| A | SER247 |
| A | ALA318 |
| A | PHE319 |
| A | HIS348 |
| A | CYS378 |
| A | ILE379 |
| A | GLY380 |
| A | HOH450 |
| A | HOH467 |
| A | HOH9750 |
| A | HOH9779 |
| A | HOH9799 |
| A | HOH9827 |
| A | HOH9835 |
| A | HOH9866 |
| A | HOH9990 |
| C | LYS208 |
| D | MET134 |
| A | LEU88 |
| A | ALA89 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SO4 B 9719 |
| Chain | Residue |
| B | LYS298 |
| B | ARG302 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SO4 B 9721 |
| Chain | Residue |
| B | SER260 |
| B | ALA262 |
| B | GLU263 |
| B | ARG266 |
| B | HOH442 |
| B | HOH451 |
| site_id | AC6 |
| Number of Residues | 29 |
| Details | BINDING SITE FOR RESIDUE CAA B 2393 |
| Chain | Residue |
| B | LEU88 |
| B | ALA89 |
| B | GLY147 |
| B | LEU148 |
| B | HIS156 |
| B | MET157 |
| B | ARG220 |
| B | SER227 |
| B | MET228 |
| B | LEU231 |
| B | ALA243 |
| B | GLY244 |
| B | SER247 |
| B | ALA318 |
| B | PHE319 |
| B | HIS348 |
| B | CYS378 |
| B | ILE379 |
| B | GLY380 |
| B | HOH417 |
| B | HOH435 |
| B | HOH479 |
| B | HOH9734 |
| B | HOH9756 |
| B | HOH9759 |
| B | HOH9777 |
| B | HOH9790 |
| B | HOH9914 |
| C | MET134 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Acyl-thioester intermediate |
| Chain | Residue | Details |
| A | ALA89 | |
| B | ALA89 | |
| C | ALA89 | |
| D | ALA89 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | ACT_SITE: Proton acceptor |
| Chain | Residue | Details |
| A | HIS348 | |
| A | CYS378 | |
| B | HIS348 | |
| B | CYS378 | |
| C | HIS348 | |
| C | CYS378 | |
| D | HIS348 | |
| D | CYS378 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| A | HIS348 | |
| A | CYS378 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| B | HIS348 | |
| B | CYS378 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| C | HIS348 | |
| C | CYS378 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1qfl |
| Chain | Residue | Details |
| D | HIS348 | |
| D | CYS378 |
