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1JZ5

E. COLI (lacZ) BETA-GALACTOSIDASE IN COMPLEX WITH D-GALCTOPYRANOSYL-1-ON

Functional Information from GO Data
ChainGOidnamespacecontents
A0000287molecular_functionmagnesium ion binding
A0003824molecular_functioncatalytic activity
A0004553molecular_functionhydrolase activity, hydrolyzing O-glycosyl compounds
A0004565molecular_functionbeta-galactosidase activity
A0005975biological_processcarbohydrate metabolic process
A0005990biological_processlactose catabolic process
A0008152biological_processmetabolic process
A0009341cellular_componentbeta-galactosidase complex
A0016787molecular_functionhydrolase activity
A0016798molecular_functionhydrolase activity, acting on glycosyl bonds
A0030246molecular_functioncarbohydrate binding
A0031420molecular_functionalkali metal ion binding
A0042802molecular_functionidentical protein binding
A0046872molecular_functionmetal ion binding
A1901575biological_processorganic substance catabolic process
B0000287molecular_functionmagnesium ion binding
B0003824molecular_functioncatalytic activity
B0004553molecular_functionhydrolase activity, hydrolyzing O-glycosyl compounds
B0004565molecular_functionbeta-galactosidase activity
B0005975biological_processcarbohydrate metabolic process
B0005990biological_processlactose catabolic process
B0008152biological_processmetabolic process
B0009341cellular_componentbeta-galactosidase complex
B0016787molecular_functionhydrolase activity
B0016798molecular_functionhydrolase activity, acting on glycosyl bonds
B0030246molecular_functioncarbohydrate binding
B0031420molecular_functionalkali metal ion binding
B0042802molecular_functionidentical protein binding
B0046872molecular_functionmetal ion binding
B1901575biological_processorganic substance catabolic process
C0000287molecular_functionmagnesium ion binding
C0003824molecular_functioncatalytic activity
C0004553molecular_functionhydrolase activity, hydrolyzing O-glycosyl compounds
C0004565molecular_functionbeta-galactosidase activity
C0005975biological_processcarbohydrate metabolic process
C0005990biological_processlactose catabolic process
C0008152biological_processmetabolic process
C0009341cellular_componentbeta-galactosidase complex
C0016787molecular_functionhydrolase activity
C0016798molecular_functionhydrolase activity, acting on glycosyl bonds
C0030246molecular_functioncarbohydrate binding
C0031420molecular_functionalkali metal ion binding
C0042802molecular_functionidentical protein binding
C0046872molecular_functionmetal ion binding
C1901575biological_processorganic substance catabolic process
D0000287molecular_functionmagnesium ion binding
D0003824molecular_functioncatalytic activity
D0004553molecular_functionhydrolase activity, hydrolyzing O-glycosyl compounds
D0004565molecular_functionbeta-galactosidase activity
D0005975biological_processcarbohydrate metabolic process
D0005990biological_processlactose catabolic process
D0008152biological_processmetabolic process
D0009341cellular_componentbeta-galactosidase complex
D0016787molecular_functionhydrolase activity
D0016798molecular_functionhydrolase activity, acting on glycosyl bonds
D0030246molecular_functioncarbohydrate binding
D0031420molecular_functionalkali metal ion binding
D0042802molecular_functionidentical protein binding
D0046872molecular_functionmetal ion binding
D1901575biological_processorganic substance catabolic process
Functional Information from PROSITE/UniProt
site_idPS00608
Number of Residues15
DetailsGLYCOSYL_HYDROL_F2_2 Glycosyl hydrolases family 2 acid/base catalyst. DRNHPSVIIWSlg.NE
ChainResidueDetails
AASP447-GLU461

site_idPS00719
Number of Residues26
DetailsGLYCOSYL_HYDROL_F2_1 Glycosyl hydrolases family 2 signature 1. NaVRCSHYPnhplWYtlcDryGLYVV
ChainResidueDetails
AASN385-VAL410

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsACT_SITE: Proton donor => ECO:0000269|PubMed:6420154
ChainResidueDetails
AGLU461
BGLU461
CGLU461
DGLU461

site_idSWS_FT_FI2
Number of Residues4
DetailsACT_SITE: Nucleophile => ECO:0000269|PubMed:1350782
ChainResidueDetails
AGLU537
BGLU537
CGLU537
DGLU537

site_idSWS_FT_FI3
Number of Residues28
DetailsBINDING:
ChainResidueDetails
AASN102
BGLU461
BGLU537
BPHE601
BASN604
BTRP999
CASN102
CASP201
CGLU461
CGLU537
CPHE601
AASP201
CASN604
CTRP999
DASN102
DASP201
DGLU461
DGLU537
DPHE601
DASN604
DTRP999
AGLU461
AGLU537
APHE601
AASN604
ATRP999
BASN102
BASP201

site_idSWS_FT_FI4
Number of Residues12
DetailsBINDING: BINDING => ECO:0000269|PubMed:11045615
ChainResidueDetails
AGLU416
DGLU416
DHIS418
DASN597
AHIS418
AASN597
BGLU416
BHIS418
BASN597
CGLU416
CHIS418
CASN597

site_idSWS_FT_FI5
Number of Residues8
DetailsSITE: Transition state stabilizer
ChainResidueDetails
AHIS357
AHIS391
BHIS357
BHIS391
CHIS357
CHIS391
DHIS357
DHIS391

site_idSWS_FT_FI6
Number of Residues4
DetailsSITE: Important for ensuring that an appropriate proportion of lactose is converted to allolactose
ChainResidueDetails
ATRP999
BTRP999
CTRP999
DTRP999

Catalytic Information from CSA
site_idMCSA1
Number of Residues10
DetailsM-CSA 422
ChainResidueDetails
AASP201
AASN604
AHIS357
AHIS391
AGLU416
AHIS418
AGLU461
AGLU537
AASN597
APHE601

site_idMCSA2
Number of Residues10
DetailsM-CSA 422
ChainResidueDetails
BASP201
BASN604
BHIS357
BHIS391
BGLU416
BHIS418
BGLU461
BGLU537
BASN597
BPHE601

site_idMCSA3
Number of Residues10
DetailsM-CSA 422
ChainResidueDetails
CASP201
CASN604
CHIS357
CHIS391
CGLU416
CHIS418
CGLU461
CGLU537
CASN597
CPHE601

site_idMCSA4
Number of Residues10
DetailsM-CSA 422
ChainResidueDetails
DASP201
DASN604
DHIS357
DHIS391
DGLU416
DHIS418
DGLU461
DGLU537
DASN597
DPHE601

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PDB entries from 2024-04-24

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