1E0T

R292D mutant of E. coli pyruvate kinase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0003824	molecular_function	catalytic activity
A	0004743	molecular_function	pyruvate kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006096	biological_process	glycolytic process
A	0008152	biological_process	metabolic process
A	0009408	biological_process	response to heat
A	0016020	cellular_component	membrane
A	0016301	molecular_function	kinase activity
A	0016310	biological_process	phosphorylation
A	0016740	molecular_function	transferase activity
A	0030955	molecular_function	potassium ion binding
A	0032869	biological_process	cellular response to insulin stimulus
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
A	1902912	cellular_component	pyruvate kinase complex
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0003824	molecular_function	catalytic activity
B	0004743	molecular_function	pyruvate kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006096	biological_process	glycolytic process
B	0008152	biological_process	metabolic process
B	0009408	biological_process	response to heat
B	0016020	cellular_component	membrane
B	0016301	molecular_function	kinase activity
B	0016310	biological_process	phosphorylation
B	0016740	molecular_function	transferase activity
B	0030955	molecular_function	potassium ion binding
B	0032869	biological_process	cellular response to insulin stimulus
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding
B	1902912	cellular_component	pyruvate kinase complex
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0003824	molecular_function	catalytic activity
C	0004743	molecular_function	pyruvate kinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006096	biological_process	glycolytic process
C	0008152	biological_process	metabolic process
C	0009408	biological_process	response to heat
C	0016020	cellular_component	membrane
C	0016301	molecular_function	kinase activity
C	0016310	biological_process	phosphorylation
C	0016740	molecular_function	transferase activity
C	0030955	molecular_function	potassium ion binding
C	0032869	biological_process	cellular response to insulin stimulus
C	0042802	molecular_function	identical protein binding
C	0046872	molecular_function	metal ion binding
C	1902912	cellular_component	pyruvate kinase complex
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0003824	molecular_function	catalytic activity
D	0004743	molecular_function	pyruvate kinase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006096	biological_process	glycolytic process
D	0008152	biological_process	metabolic process
D	0009408	biological_process	response to heat
D	0016020	cellular_component	membrane
D	0016301	molecular_function	kinase activity
D	0016310	biological_process	phosphorylation
D	0016740	molecular_function	transferase activity
D	0030955	molecular_function	potassium ion binding
D	0032869	biological_process	cellular response to insulin stimulus
D	0042802	molecular_function	identical protein binding
D	0046872	molecular_function	metal ion binding
D	1902912	cellular_component	pyruvate kinase complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 701

Chain	Residue
A	THR378
A	GLN379
A	GLY380
A	GLY381
A	LYS382
A	SER383
A	GLY460

---

site_id	AC2
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 B 702

Chain	Residue
B	GLY380
B	GLY381
B	LYS382
B	SER383
B	SER459
B	GLY460
B	THR378
B	GLN379

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 C 703

Chain	Residue
C	THR378
C	GLN379
C	GLY380
C	GLY381
C	LYS382
C	SER459
C	GLY460

---

site_id	AC4
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 D 704

Chain	Residue
D	THR378
D	GLN379
D	GLY380
D	GLY381
D	LYS382
D	SER383
D	SER459
D	GLY460
D	THR461

---

Functional Information from PROSITE/UniProt

site_id	PS00110
Number of Residues	13
Details	PYRUVATE_KINASE Pyruvate kinase active site signature. IhIISKIENqEGL

Chain	Residue	Details
A	ILE215-LEU227

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	40
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P30613

Chain	Residue	Details
A	ARG32
A	THR278
B	ARG32
B	ASN34
B	SER36
B	ASP66
B	THR67
B	LYS220
B	GLU222
B	GLY245
B	ASP246
A	ASN34
B	THR278
C	ARG32
C	ASN34
C	SER36
C	ASP66
C	THR67
C	LYS220
C	GLU222
C	GLY245
C	ASP246
A	SER36
C	THR278
D	ARG32
D	ASN34
D	SER36
D	ASP66
D	THR67
D	LYS220
D	GLU222
D	GLY245
D	ASP246
A	ASP66
D	THR278
A	THR67
A	LYS220
A	GLU222
A	GLY245
A	ASP246

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P14618

Chain	Residue	Details
A	ARG73
A	LYS156
B	ARG73
B	LYS156
C	ARG73
C	LYS156
D	ARG73
D	LYS156

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	SITE: Transition state stabilizer => ECO:0000250|UniProtKB:P00549

Chain	Residue	Details
A	LYS220
B	LYS220
C	LYS220
D	LYS220

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:18723842

Chain	Residue	Details
A	LYS76
A	LYS319
B	LYS76
B	LYS319
C	LYS76
C	LYS319
D	LYS76
D	LYS319

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