8WVF

Crystal structure of Lsd18 mutant T189M and S195M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-03
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	63.005, 48.116, 135.569
Unit cell angles	90.00, 91.56, 90.00

Refinement procedure

Resolution	20.044 - 3.757
R-factor	0.2365
Rwork	0.232
R-free	0.31910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8UP4
RMSD bond length	0.002
RMSD bond angle	0.565
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.044	3.897
High resolution limit [Å]	3.757	3.757
Rmerge	0.268	0.389
Rmeas	0.317	0.473
Rpim	0.165	0.266
Number of reflections	7514	689
<I/σ(I)>	3.7	2.2
Completeness [%]	88.0
Redundancy	1
CC(1/2)	0.870	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.2 M Li2SO4, 0.1M Tris, pH 7.5, 30% PEG4000