8WRA
The Crystal Structure of CASP1 from Biortus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 62.807, 62.807, 144.132 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.394 - 1.450 |
Rwork | 0.192 |
R-free | 0.21290 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.087 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.040 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.101 | 1.042 |
Number of reflections | 52141 | 2526 |
<I/σ(I)> | 17.6 | 2.6 |
Completeness [%] | 100.0 | |
Redundancy | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.15M Na formate, 14% PEG3350 |