8V14
Structure of NRAP-1 and its role in NMDAR signaling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.358, 75.921, 152.381 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.120 - 1.900 |
| R-factor | 0.1954 |
| Rwork | 0.194 |
| R-free | 0.22890 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.828 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.120 | 38.100 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.111 | 0.047 | 5.366 |
| Rmeas | 0.112 | 0.047 | 5.420 |
| Rpim | 0.015 | 0.007 | 0.759 |
| Total number of observations | 19511 | 122697 | |
| Number of reflections | 38804 | 431 | 2443 |
| <I/σ(I)> | 27.4 | 81.2 | 1.2 |
| Completeness [%] | 100.0 | 99.2 | 100 |
| Redundancy | 54.8 | 45.3 | 50.2 |
| CC(1/2) | 1.000 | 0.999 | 0.822 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.35 | 277 | 17 mg/mL purified NRAP-1 in 150 mM sodium chloride, 15 mM HEPES, 1 mM calcium chloride, pH 7.35, 1:1 with crystallization solution (26% PEG8000, 100 mM CHES, pH 9.8) |
