8USO
Full-length human CaMKII delta holoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 174.984, 174.984, 140.416 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.530 - 2.300 |
| R-factor | 0.1851 |
| Rwork | 0.184 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.503 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.158 | 0.046 | 1.626 |
| Rmeas | 0.162 | 0.048 | 1.691 |
| Rpim | 0.036 | 0.011 | 0.446 |
| Total number of observations | 1932274 | ||
| Number of reflections | 96528 | 5189 | 4754 |
| <I/σ(I)> | 5.3 | ||
| Completeness [%] | 100.0 | 99.9 | 99.9 |
| Redundancy | 20 | 19.9 | 12.9 |
| CC(1/2) | 0.992 | 0.997 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M pH 6.5 Bis-Tris, 16% PEG 2K , 0.2 M sodium malonate dibasic |
