8UPI
Structure of a periplasmic peptide binding protein from Mesorhizobium sp. AP09 bound to aminoserine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-06 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.247, 58.920, 139.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.510 - 1.550 |
| R-factor | 0.1815 |
| Rwork | 0.179 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.801 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.510 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.078 | 0.503 |
| Rmeas | 0.089 | 0.634 |
| Rpim | 0.041 | 0.378 |
| Total number of observations | 254272 | 9261 |
| Number of reflections | 67507 | 3194 |
| <I/σ(I)> | 7.8 | 2.1 |
| Completeness [%] | 99.2 | |
| Redundancy | 3.8 | 2.9 |
| CC(1/2) | 0.985 | 0.445 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30% PEG 4000 |
