8UH1

Crystal structure of T. brucei EIF4E6 in complex with EIF4G5 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	C 2 2 21
Unit cell lengths	65.503, 131.185, 59.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.000 - 1.898
R-factor	0.1942
Rwork	0.192
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	16430	822
<I/σ(I)>	13.2
Completeness [%]	95.1
Redundancy	13.3
CC(1/2)	0.990	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	Complex at 12 mg/mL in 20 mM Tris-HCl, 150 mM NaCl, 2 mM DTT, pH 8.0 (pre-incubated with cap-4) with crystallization buffer containing 33% Precipitant Mix 4, 0.09 M NPS, 0.12 M Ethylene glycols, 0.1 M Buffer System 3 (pH 8.5), from Morpheus Screen mixes