8UCY

Sterile Alpha Motif (SAM) domain from Tric1, Arabidopsis thaliana

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-09-25
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	28.639, 92.121, 93.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.170 - 1.480
R-factor	0.1801
Rwork	0.179
R-free	0.19810
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.163
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200	1.510
High resolution limit [Å]	1.480	1.480
Rmerge	0.055	1.310
Rpim	0.015	0.403
Number of reflections	42224	2085
<I/σ(I)>	28.1	1.8
Completeness [%]	99.9	99.1
Redundancy	14.3	11.1
CC(1/2)	1.000	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	1.5-2.0 M ammonium sulphate, 0.1 M Tris