8SZT

Structure of Kdac1 from Acinetobacter baumannii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	113.949, 181.689, 155.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.510 - 2.500
R-factor	0.2031
Rwork	0.201
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.942
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	96.530	2.570
High resolution limit [Å]	2.500	2.500
Number of reflections	55541	36436
<I/σ(I)>	7.4	2.9
Completeness [%]	99.6
Redundancy	6.5
CC(1/2)	0.969	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	Kdac1 10mg/mL 0.6 M sodium formate and 20% (w/v) polyethylene glycol 3,350 Seeded