8SZR

Dog DHX9 bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95364
Spacegroup name	P 43 21 2
Unit cell lengths	85.290, 85.290, 352.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.350 - 2.970
Rwork	0.243
R-free	0.29580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.814
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.350	3.150
High resolution limit [Å]	2.970	2.970
Rmerge	0.066	1.301
Rmeas	0.067	1.327
Rpim	0.013	0.259
Total number of observations	734995	113359
Number of reflections	27977	4412
<I/σ(I)>	33	3.2
Completeness [%]	100.0
Redundancy	26.3	25.7
CC(1/2)	1.000	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	200 mM Lithium sulfate, 100 mM MES pH 6, 20% (w/v) PEG 4000; 5 mM ADP and 50 mM magnesium chloride in protein solution