8SKI
Crystal structure of human DNA polymerase eta incorporating 5F-dUTP across HX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2023-02-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979338 |
Spacegroup name | P 61 |
Unit cell lengths | 98.560, 98.560, 81.094 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.260 - 2.163 |
R-factor | 0.2021 |
Rwork | 0.200 |
R-free | 0.24930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o3n |
RMSD bond length | 0.009 |
RMSD bond angle | 1.105 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.791 | 2.202 |
High resolution limit [Å] | 2.163 | 2.163 |
Number of reflections | 24004 | 1260 |
<I/σ(I)> | 9.665 | |
Completeness [%] | 100.0 | |
Redundancy | 13.43 | |
CC(1/2) | 0.998 | 0.481 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MES, pH 6.5, 20% PEG2000 MME, 5 mM calcium chloride |