8SD7
Carbonic anhydrase II radiation damage RT 61-90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | PAL-XFEL BEAMLINE CSI |
| Synchrotron site | PAL-XFEL |
| Beamline | CSI |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2018-11-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.713, 41.578, 72.707 |
| Unit cell angles | 90.00, 104.44, 90.00 |
Refinement procedure
| Resolution | 24.015 - 1.704 |
| R-factor | 0.1326 |
| Rwork | 0.132 |
| R-free | 0.15300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ks3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.033 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.132 | 0.156 |
| Number of reflections | 27126 | 27126 |
| <I/σ(I)> | 16.96 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 1.6 M sodium citrate, 50 mM Tris base |
