8RM6
Crystal Structure of Human Androgen Receptor DNA Binding Domain Bound to its Response Element: C3(1)ARE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-14 |
Detector | DECTRIS PILATUS3 X 2M |
Wavelength(s) | 0.8731 |
Spacegroup name | P 2 21 2 |
Unit cell lengths | 37.029, 95.359, 115.353 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.350 - 2.050 |
R-factor | 0.2225 |
Rwork | 0.221 |
R-free | 0.24680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.050 |
Data reduction software | XDS (DECEMBER 2003 VERSION) |
Data scaling software | Aimless (CCP4Interface 7.1.018) |
Phasing software | PHASER (CCP4Interface 7.1.018) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.350 | 2.110 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.239 | 2.509 |
Rmeas | 0.242 | 2.539 |
Rpim | 0.033 | 0.386 |
Number of reflections | 26537 | 1972 |
<I/σ(I)> | 19.9 | 2.5 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 51.3 | 42.8 |
CC(1/2) | 0.999 | 0.821 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1M sodium hepes pH7.5,15% (w/v) peg 20000 |