8Q66
Crystal Structure of the C. elegans MUT-7 MUT-8 CTD complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-26 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.87313 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.089, 59.053, 88.161 |
Unit cell angles | 90.00, 119.80, 90.00 |
Refinement procedure
Resolution | 67.910 - 2.030 |
R-factor | 0.1925 |
Rwork | 0.191 |
R-free | 0.22470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.561 |
Data reduction software | XDS (20230630) |
Data scaling software | pointless |
Phasing software | PHENIX (1.12.1-4487) |
Refinement software | PHENIX (1.12.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.910 | 2.103 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.088 | |
Number of reflections | 42083 | 4152 |
<I/σ(I)> | 9.1 | |
Completeness [%] | 99.7 | |
Redundancy | 3.4 | |
CC(1/2) | 0.997 | 0.410 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 30 %w/v EDO_P8K (Precipitant) 0.1 M MB3 8.5 pH (Buffer) 0.06 M divalent (Complex ingredient) |