8PZ3
TssM - A USP-like DUB from B. pseudomallei (193-430)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91840 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 116.850, 116.850, 111.320 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.600 - 3.150 |
R-factor | 0.2336 |
Rwork | 0.229 |
R-free | 0.27530 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.946 |
Data reduction software | XDS (BUILT=20210323) |
Data scaling software | XSCALE (BUILT=20210323) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18rc4_3812) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.880 | 3.230 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmeas | 0.250 | 1.079 |
Number of reflections | 15249 | 1109 |
<I/σ(I)> | 5.75 | 1.53 |
Completeness [%] | 97.6 | 96.9 |
Redundancy | 4.69 | 4.76 |
CC(1/2) | 0.985 | 0.523 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.15 M ammonium chloride, 22 % w/v PEG 3350 and 4 % Dextran sulfate sodium salt Mr 5,000; 1:2, 1:1, 2:1 protein reservoir drop ration; cryoprotected with reservoir + 25% Glycerol |