8P9O
PCNA from Chaetomium thermophilum in complex with PolD3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2023-02-20 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 55.840, 56.391, 62.860 |
Unit cell angles | 90.93, 90.55, 99.48 |
Refinement procedure
Resolution | 27.570 - 2.450 |
R-factor | 0.2144 |
Rwork | 0.213 |
R-free | 0.24943 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.962 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.570 | 2.550 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.091 | 0.255 |
Rmeas | 0.113 | 0.324 |
Rpim | 0.067 | 0.197 |
Total number of observations | 66712 | 6953 |
Number of reflections | 25893 | 2840 |
<I/σ(I)> | 5.7 | 2.8 |
Completeness [%] | 93.2 | |
Redundancy | 2.6 | 2.4 |
CC(1/2) | 0.988 | 0.881 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.1 M Phosphate/citrate, pH4.2, 40% v/v PEG300 |