8P35
Mutant human titin immunoglobulin-like 21 domain - C3575S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.185, 64.892, 288.038 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.210 - 2.200 |
Rwork | 0.220 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.210 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.080 | 0.400 |
Rpim | 0.024 | 0.116 |
Number of reflections | 35855 | 3088 |
<I/σ(I)> | 22.6 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 24.5 | |
CC(1/2) | 0.995 | 0.984 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES sodium salt, pH 7.5, 25 %(w/v) PEG 2000 MME |