8OU0
bovine sperm endpiece singlet microtubules (one tubulin dimer and associated microtubule inner proteins)
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TALOS ARCTICA |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.5 |
Software | RELION,SerialEM,Gctf |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |