8OIJ

Drosophila Smaug-Smoothened complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976254
Spacegroup name	P 61 2 2
Unit cell lengths	76.590, 76.590, 268.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.390 - 2.000
R-factor	0.2737
Rwork	0.272
R-free	0.29250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.528
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.330	2.230
High resolution limit [Å]	2.000	2.000
Number of reflections	21810	1090
<I/σ(I)>	7
Completeness [%]	94.0
Redundancy	9.9
CC(1/2)	0.996	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1M HEPES pH 7.5, 5 % (w/v) PEG 3000, 20 % (w/v) PEG 400