8OGB
Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-18 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 85.018, 85.018, 219.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.206 - 2.270 |
Rwork | 0.168 |
R-free | 0.21250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 2.049 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 219.360 | 2.340 |
High resolution limit [Å] | 2.270 | 2.270 |
Number of reflections | 22640 | 2016 |
<I/σ(I)> | 14.8 | |
Completeness [%] | 100.0 | |
Redundancy | 18.8 | |
CC(1/2) | 0.998 | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Tris pH 7.5, 2.13M Lithium acetate |