8KGZ
Crystal structure of single-chain Fv antibody against antigen peptide from SARS-CoV2 S-spike protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-01-01 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.210, 77.753, 61.346 |
Unit cell angles | 90.00, 112.25, 90.00 |
Refinement procedure
Resolution | 55.790 - 2.210 |
R-factor | 0.27129 |
Rwork | 0.269 |
R-free | 0.32114 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.930 |
Data reduction software | HKL-3000 |
Data scaling software | DIALS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.750 | 2.270 |
High resolution limit [Å] | 2.210 | 2.210 |
Number of reflections | 179188 | 26361 |
<I/σ(I)> | 4.1 | |
Completeness [%] | 100.0 | |
Redundancy | 6.5 | 6.4 |
CC(1/2) | 0.882 | 0.882 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 25% PEG 3350, 0.1M HEPES pH7.5 |