8K3K
The crystal structure of nanobody Nb4 in complex with receptor binding domain (RBD) of BA.1 Spike protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL19U1 |
Synchrotron site | NFPSS |
Beamline | BL19U1 |
Temperature [K] | 77.15 |
Detector technology | PIXEL |
Collection date | 2022-08-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 76.110, 76.110, 251.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.370 - 2.430 |
R-factor | 0.2286 |
Rwork | 0.224 |
R-free | 0.26920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.633 |
Data reduction software | AUTOMAR |
Data scaling software | xia2 |
Phasing software | PHENIX (1.19.2-4158) |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.370 | 2.490 |
High resolution limit [Å] | 2.430 | 2.430 |
Number of reflections | 30440 | 828 |
<I/σ(I)> | 14.8 | |
Completeness [%] | 100.0 | |
Redundancy | 36.8 | |
CC(1/2) | 0.997 | 0.706 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M potassium thiocyanate, 20 %(w/v) PEG 3350 |