8JQY
Crystal Structure of nucleotide-free mIRGB10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-05-03 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.97957 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 190.240, 190.240, 38.880 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.610 - 3.680 |
R-factor | 0.2506 |
Rwork | 0.249 |
R-free | 0.27190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.573 |
Data reduction software | XDS |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.610 | 3.812 |
High resolution limit [Å] | 3.680 | 3.681 |
Number of reflections | 8893 | 891 |
<I/σ(I)> | 18.54 | |
Completeness [%] | 99.7 | 100 |
Redundancy | 20.7 | |
CC(1/2) | 0.999 | 0.671 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M ammonium sulfate, 0.2 M lithium sulfate, and 0.1 M Tris-HCl (pH 7.0) |