8IXQ
Structure of glycosyltransferase LmbT in complex with GDP and ergothioneine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-16 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.634, 139.484, 158.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.820 - 2.100 |
| R-factor | 0.1843 |
| Rwork | 0.184 |
| R-free | 0.22050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.896 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.820 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.115 | 0.712 |
| Number of reflections | 126789 | 6208 |
| <I/σ(I)> | 11.8 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 8 |
| CC(1/2) | 0.998 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.03M citrate acid, 0.07M BIS-tris propane (pH7.6), 28% Polyethylene glycol 3,350 |
