8IXP
Apo structure of glycosyltransferase LmbT wild type
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-11 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.536, 142.015, 158.867 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.770 - 2.450 |
R-factor | 0.2045 |
Rwork | 0.203 |
R-free | 0.25360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.883 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.770 | 2.500 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.155 | 1.080 |
Number of reflections | 81741 | 4453 |
<I/σ(I)> | 12.8 | 2.2 |
Completeness [%] | 100.0 | |
Redundancy | 13.6 | |
CC(1/2) | 0.998 | 0.879 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.03M citrate acid, 0.07M BIS-tris propane pH7.6, 28% Polyethylene glycol 3,350 |