8IU9

Crystal structure of GH66 endodextranase from Flavobacterium johnsoniae in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	155.181, 48.381, 76.487
Unit cell angles	90.00, 104.41, 90.00

Refinement procedure

Resolution	47.256 - 1.800
Rwork	0.206
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.165
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.260	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.132	0.963
Rmeas	0.156	1.133
Rpim	0.083	0.591
Number of reflections	50429	50429
<I/σ(I)>	10.3	2.1
Completeness [%]	98.3
Redundancy	6.7
CC(1/2)	0.997	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM Tris-HCl, pH 8.5-9.0, 200mM lithium sulfate, 20% PEG 4000