8IQC
Crystal structure of AsfvPrimPol N-terminal Prim/Pol domain in complex with Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.424, 43.922, 87.584 |
| Unit cell angles | 90.00, 93.29, 90.00 |
Refinement procedure
| Resolution | 29.550 - 2.000 |
| R-factor | 0.2467 |
| Rwork | 0.244 |
| R-free | 0.27720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.090 | 0.086 | 0.455 |
| Number of reflections | 44549 | 4677 | 4072 |
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 98.5 | 99.9 | 90.9 |
| Redundancy | 5 | 6.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Bis-Tris propane pH 7.5, 25% PEG 3350 and 0.2 M Sodium citrate |
