8IPZ
Crystal structure of insulin detemir
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-07 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.5 |
| Spacegroup name | H 3 |
| Unit cell lengths | 78.874, 78.874, 79.282 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.770 - 1.400 |
| R-factor | 0.1945 |
| Rwork | 0.192 |
| R-free | 0.22970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.480 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.770 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 36231 | 2608 |
| <I/σ(I)> | 13.91 | |
| Completeness [%] | 99.8 | |
| Redundancy | 1 | |
| CC(1/2) | 0.990 | 0.210 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M sodium acetate trihydrate, 0.1M TRIS hydrochloride |
